Hill E M, Holland D L, East J
School of Ocean Sciences, University College of North Wales, Anglesey, UK.
Biochim Biophys Acta. 1993 Jul 11;1157(3):297-303. doi: 10.1016/0304-4165(93)90113-m.
Previous work has shown that eicosanoid hatching factors can be isolated from tissues of a variety of barnacle species. To further investigate the nature of barnacle hatching factor an eicosanoid fraction isolated from homogenates of the barnacle Balanus balanoides was purified on RP-HPLC and trihydroxy fatty acid fractions were bioassayed for hatching activity and analysed by GC-MS. The identified eicosanoids were 8,11,12-trihydroxy-5,9,14-eicosatrienoic acid, 8,11,12-trihydroxy-5,9,14,17-eicosatetraenoic acid, 10,11,12-trihydroxy-5,8,14,17-eicosatetraenoic acid and 10,13, 14-trihydroxy-4,7,11,16,19-docosapentaenoic acid. These studies suggest that barnacles possess a potent n-9 lipoxygenase which can metabolise either arachidonic acid, eicosapentaenoic acid or docosahexaenoic acid. The predominant eicosanoids common to all active fractions were either 8,11,12-trihydroxyeicosatrienoic acid or an isomer of 8,11-12-trihydroxyeicosatetraenoic acid, which suggests that a particular configuration of an 8,11,12 triol is barnacle hatching factor.
先前的研究表明，类二十烷酸孵化因子可以从多种藤壶物种的组织中分离出来。为了进一步研究藤壶孵化因子的性质，从藤壶龟甲藤壶匀浆中分离出的类二十烷酸馏分在反相高效液相色谱上进行纯化，对三羟基脂肪酸馏分进行孵化活性生物测定，并通过气相色谱 - 质谱联用仪进行分析。鉴定出的类二十烷酸为8,11,12 - 三羟基 - 5,9,14 - 二十碳三烯酸、8,11,12 - 三羟基 - 5,9,14,17 - 二十碳四烯酸、10,11,12 - 三羟基 - 5,8,14,17 - 二十碳四烯酸和10,13,14 - 三羟基 - 4,7,11,16,19 - 二十二碳五烯酸。这些研究表明，藤壶拥有一种强大的n - 9脂氧合酶，它可以代谢花生四烯酸、二十碳五烯酸或二十二碳六烯酸。所有活性馏分共有的主要类二十烷酸要么是8,11,12 - 三羟基二十碳三烯酸，要么是8,11 - 12 - 三羟基二十碳四烯酸的异构体，这表明8,11,12 - 三醇的特定构型是藤壶孵化因子。
