Xie Xiuchao, Chen Rong-Xin, Song Yu, Lin Chao, Zhang Ming, Li Minglei, Qi Jianzhao
Shaanxi Province Key Laboratory of Bio-Resources, Qinba State Key Laboratory of Biological Resources and Ecological Environment (Incubation), School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China.
Agricultural Technology Extension Center of Liuba County, Liuba, Shaanxi 724100, China.
Genomics. 2025 May;117(3):111057. doi: 10.1016/j.ygeno.2025.111057. Epub 2025 May 10.
Trichaptum biforme is a white-rot fungus that plays a key role in the process of cellulose degradation. In this study, we employed a suite of techniques to sequence the genome of T. biforme, and achieved high-quality assembly and detailed annotation. The genome spans 50.71 Mb, comprises 13 chromosomal pseudomolecules, and encodes 15,302 predicted genes, exhibiting a BUSCO completeness of 96.30 %. Comparative genomic analysis has elucidated the similarities in gene composition and the differences in evolutionary pressure between T. biforme and T. abietinum. Phylogenetic analysis revealed the evolutionary position of T. abietinum and indicates that their divergence time is 21.20 million years ago (MYAs). Bioinformatic analysis revealed 375 genes encoding carbohydrate-active enzymes (CAZymes), of which 144 CAZymes were predicted to interact with 18 polysaccharide synthases. In conclusion, this work reports for the first time the genome of T. biforme, providing a comprehensive understanding of its complex functions, and elucidating the genetic basis of its ability to degrade lignocellulose.
双色拟层孔菌是一种在纤维素降解过程中起关键作用的白腐真菌。在本研究中，我们采用了一系列技术对双色拟层孔菌的基因组进行测序，并实现了高质量的组装和详细注释。该基因组跨度为50.71 Mb，由13条染色体假分子组成，编码15,302个预测基因，BUSCO完整性为96.30%。比较基因组分析阐明了双色拟层孔菌和冷杉拟层孔菌在基因组成上的相似性以及进化压力上的差异。系统发育分析揭示了冷杉拟层孔菌的进化位置，并表明它们的分化时间为2120万年前。生物信息学分析揭示了375个编码碳水化合物活性酶（CAZymes）的基因，其中144个CAZymes预计与18种多糖合酶相互作用。总之，这项工作首次报道了双色拟层孔菌的基因组，全面了解了其复杂功能，并阐明了其降解木质纤维素能力的遗传基础。
