Rofstad E K, Wahl A, Tveit K M, Monge O R, Brustad T
Radiother Oncol. 1985 Aug;4(1):33-44. doi: 10.1016/s0167-8140(85)80060-8.
X-ray and heat survival curves were established for melanoma cells derived directly from surgical specimens of tumours in man by using the Courtenay soft agar colony assay. The plating efficiency for 11 of the 14 melanomas studied was sufficiently high (PE = 0.3-58%) to measure cell survival over at least two decades. Experiments repeated with cells stored in liquid nitrogen showed that the survival assay gave highly reproducible results. The melanomas exhibited individual and characteristic survival curves whether exposed to radiation or heat (43.5 degrees C). The Do-values were in the ranges 0.63-1.66 Gy (X-rays) and 33-58 min (heat). The survival curves were similar to those reported previously for human melanoma xenografts. The radiation sensitivity of the cells was not correlated to the heat sensitivity. Since the melanomas appeared to be very heterogeneous in radiation response in vitro as melanomas are known to be clinically, it is suggested that melanomas may be suitable for prospective studies aimed at establishing whether clinical radioresponsiveness somehow is related to in vitro survival curve parameters.
通过使用考特尼软琼脂集落测定法，为直接取自人类肿瘤手术标本的黑色素瘤细胞建立了X射线和热存活曲线。在所研究的14例黑色素瘤中，有11例的接种效率足够高（PE = 0.3 - 58%），能够在至少两个数量级上测量细胞存活情况。对液氮保存的细胞进行重复实验表明，存活测定结果具有高度可重复性。无论暴露于辐射还是热（43.5摄氏度），黑色素瘤都呈现出各自独特的存活曲线。Do值范围为0.63 - 1.66 Gy（X射线）和33 - 58分钟（热）。这些存活曲线与先前报道的人类黑色素瘤异种移植的存活曲线相似。细胞的辐射敏感性与热敏感性无关。由于黑色素瘤在体外的辐射反应似乎非常异质，正如已知其在临床上的情况一样，因此建议黑色素瘤可能适合进行前瞻性研究，旨在确定临床放射反应性是否以某种方式与体外存活曲线参数相关。
