Rofstad E K, Brustad T
Br J Radiol. 1984 Nov;57(683):1023-32. doi: 10.1259/0007-1285-57-683-1023.
The response to heat (42.5 degrees C) of five human melanoma xenografts was studied. Tumours grown subcutaneously in the hind leg of athymic mice were heated in a water-bath and specific growth delay was used as a measure of response. In other experiments, cells from the same xenografts were heated in vitro and the colony-forming ability was assayed in soft agar. The slopes of the in-vivo dose-response curves (specific growth delay versus heating time) varied within a factor of about three among the five melanomas. The Do values of the in-vitro heat survival curves ranged from 44 +/- 3 to 123 +/- 15 min. The response to heat in vivo was not positively correlated with the tumour volume-doubling time, the growth fraction, the cell loss factor or the intrinsic heat sensitivity of the tumour cells, i.e., the Do values of the in vitro heat survival curves. If the results obtained in the present work are representative for clinical practice, they indicate that the response to heat may vary considerably among tumours in different patients. This variability can probably not be predicted from measurements of cytokinetic parameters of the tumours. The lack of correlation between the response to heat in vivo and in vitro demonstrates that extrapolations of results from studies in vitro to tumours are highly speculative and, when attempted, should be executed only with extreme caution.
研究了5种人黑色素瘤异种移植瘤对热（42.5摄氏度）的反应。将在无胸腺小鼠后腿皮下生长的肿瘤置于水浴中加热，并将特定生长延迟用作反应的衡量指标。在其他实验中，将来自相同异种移植瘤的细胞在体外加热，并在软琼脂中测定集落形成能力。在这5种黑色素瘤中，体内剂量反应曲线（特定生长延迟与加热时间）的斜率变化幅度约为3倍。体外热存活曲线的Do值范围为44±3至123±15分钟。体内对热的反应与肿瘤体积倍增时间、生长分数、细胞丢失因子或肿瘤细胞的内在热敏感性（即体外热存活曲线的Do值）无正相关。如果本研究获得的结果代表临床实际情况，则表明不同患者的肿瘤对热的反应可能差异很大。这种变异性可能无法通过肿瘤细胞动力学参数的测量来预测。体内和体外对热反应之间缺乏相关性表明，从体外研究结果推断肿瘤情况极具推测性，若要尝试，必须极其谨慎。
