针对16S核糖体RNA、 以及 的聚合酶链反应，用于检测怀孕35至37周女性中的B族链球菌。 （注：原文中存在部分缺失内容，用“ ”表示了）
PCR targeting 16S rRNA, , and for group B Streptococcus detection in women with 35 to 37 weeks of pregnancy.
作者信息
Liu Juan, Yan Jun, Lv Juan
机构信息
Department of Clinical Laboratory, Guangdong Provincial People's Hospital, Zhuhai Hospital (Jinwan Central Hospital of Zhuhai), Zhuhai, China.
出版信息
Biomark Med. 2025 May;19(10):379-384. doi: 10.1080/17520363.2025.2496132. Epub 2025 May 12.
AIMS
Polymerase chain reaction (PCR) is a rapid molecular technique for pathogen detection, but its application in detecting group B Streptococcus (GBS) requires further exploration. This study assessed the diagnostic performance of the PCR method for GBS detection in pregnant women with 35 to 37 weeks of pregnancy.
METHODS
This study enrolled 116 women at 35-37 weeks of pregnancy and simultaneously collected two vaginal swab samples for GBS detection via culture and PCR methods.
RESULTS
Through the culture method, 21 (18.1%) samples were positive for GBS. Moreover, 24 (20.7%), 23 (19.8%), and 23 (19.8%) samples were positive for GBS according to PCR methods targeting 16S rRNA, cfb, and hylB, respectively. The results of PCR-based GBS detection of 16S rRNA (Kappa value = 0.917, < 0.01), cfb (Kappa value = 0.944, < 0.01), and hylB (Kappa value = 0.944, < 0.01) were consistent with those of the culture method. The sensitivity and specificity were 1.000 and 0.968 for the PCR method targeting 16S rRNA, 1.000 and 0.979 for cfb, and 1.000 and 0.979 for hylB, respectively.
CONCLUSION
PCR targeting 16S rRNA, , and may serve as a reliable diagnostic method for GBS detection in women with 35 to 37 weeks of pregnancy.
目的
聚合酶链反应（PCR）是一种用于病原体检测的快速分子技术，但其在检测B族链球菌（GBS）中的应用仍需进一步探索。本研究评估了PCR方法在检测妊娠35至37周孕妇GBS中的诊断性能。
方法
本研究纳入了116名妊娠35 - 37周的女性，并同时采集两份阴道拭子样本，分别通过培养法和PCR法进行GBS检测。
结果
通过培养法，21份（18.1%）样本GBS呈阳性。此外，根据针对16S rRNA、cfb和hylB的PCR方法，分别有24份（20.7%）、23份（19.8%）和23份（19.8%）样本GBS呈阳性。基于PCR的GBS检测中，针对16S rRNA（Kappa值 = 0.917，P < 0.01）、cfb（Kappa值 = 0.944，P < 0.01）和hylB（Kappa值 = 0.944，P < 0.01）的结果与培养法一致。针对16S rRNA的PCR方法的敏感性和特异性分别为1.000和0.968，cfb为1.000和0.979，hylB为1.000和0.979。
结论
针对16S rRNA、cfb和hylB的PCR可作为检测妊娠35至37周女性GBS的可靠诊断方法。
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