Shahid Maha, Iftikhar Kanwal, Simjee Shabana Usman
Hussain Ebrahim Jamal Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
Discoveries (Craiova). 2025 Mar 31;13(1):e206. doi: 10.15190/d.2025.5. eCollection 2025 Jan-Mar.
Triton X-100 is a cell-permeabilizing agent, widely used in molecular studies to enhance the permeability of cellular membranes. It has detrimental effects on the cell surface receptors thereby not suitable for surface receptor protein studies. Therefore, in the present study the protein expression of Notch surface receptor was analyzed with and without exposure of cell surfactant i.e. Triton X-100. It was observed that cultured cortical cells treated with Triton X-100 gave false protein expression due to the disruption of cellular membrane. On the contrary, cells without surfactant treatment exhibit fluorescence proportional to the true presence of Notch 1 receptors. This method can be followed for the analysis of any surface receptor protein through immunofluorescence to exclude any false expression.
曲拉通X-100是一种细胞通透剂，广泛应用于分子研究中以增强细胞膜的通透性。它对细胞表面受体有不良影响，因此不适用于表面受体蛋白研究。所以，在本研究中，分析了有或无细胞表面活性剂（即曲拉通X-100）处理时Notch表面受体的蛋白表达情况。观察到用曲拉通X-100处理的培养皮质细胞由于细胞膜的破坏而出现假蛋白表达。相反，未经表面活性剂处理的细胞显示出与Notch 1受体真实存在成比例的荧光。通过免疫荧光分析任何表面受体蛋白时可采用此方法以排除任何假表达。
