Lee Seul Ah, Lee Seul Bi, Kim Do Kyung, Lee Sook-Young, Kim Chun Sung
Department of Oral Biochemistry, College of Dentistry, Chosun University, Gwangju 61452, Korea.
Marine Healthcare Research & Evaluation Center, Chosun University, Jeonnam 59146, Korea.
Prev Nutr Food Sci. 2025 Apr 30;30(2):141-151. doi: 10.3746/pnf.2025.30.2.141.
Osteoarthritis (OA), a degenerative disease characterized by cartilage degradation and inflammation, occurs due to trauma caused by external stimuli or cartilage aging. is a red alga that belongs to the Porphyra family and is consumed as food in Asia, especially Korea, Japan, and China. contains various bioactive substances, including carotenoids, flavonoids, and vitamins, that exert anti-inflammatory, antioxidant, and anti-photoaging effects. In the present study, the anti-osteoarthritic effects of 30% fermented alcohol extract of (30% FEPY) on interleukin-1 beta (IL-1β)-stimulated chondrocytes and a destabilization of the medial meniscus (DMM)-induced OA rat model were investigated. The results showed that pretreatment with 30% FEPY significantly reduced the IL-1β-induced expression of inflammatory factors (e.g., inducible nitric oxide synthase and cyclooxygenase-2) and cartilage-degrading enzymes [matrix metalloproteinase (MMP) 1, MMP3, MMP13, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) 4, and ADAMTS5], which was analyzed using Griess reaction, enzyme-linked immunosorbent assay, and Western blot analysis. The anti-osteoarthritic effects of 30% FEPY, which were mediated through mitogen-activated protein kinase and nuclear factor kappa-light-chain-enhancer of activated B cell signaling, were analyzed using Western blot analysis. In an study, Safranin O staining and immunohistochemistry analysis revealed that treatment with 30% FEPY significantly increased cartilage degradation and collagen type II protein expression in the DMM group. These findings collectively suggest that 30% FEPY is a promising candidate for alleviating OA progression and developing new therapeutic drugs.
骨关节炎（OA）是一种以软骨降解和炎症为特征的退行性疾病，由外部刺激或软骨老化引起的创伤所致。紫菜是一种属于红毛菜科的红藻，在亚洲尤其是韩国、日本和中国被作为食物食用。紫菜含有多种生物活性物质，包括类胡萝卜素、黄酮类化合物和维生素，具有抗炎、抗氧化和抗光老化作用。在本研究中，研究了30%紫菜发酵酒精提取物（30% FEPY）对白细胞介素-1β（IL-1β）刺激的软骨细胞和内侧半月板不稳定（DMM）诱导的OA大鼠模型的抗骨关节炎作用。结果表明，用30% FEPY预处理可显著降低IL-1β诱导的炎症因子（如诱导型一氧化氮合酶和环氧化酶-2）和软骨降解酶[基质金属蛋白酶（MMP）1、MMP3、MMP13、含血小板反应蛋白基序的解聚素和金属蛋白酶（ADAMTS）4和ADAMTS5]的表达，采用Griess反应、酶联免疫吸附测定和蛋白质印迹分析进行分析。使用蛋白质印迹分析了通过丝裂原活化蛋白激酶和活化B细胞核因子κ轻链增强子信号介导的30% FEPY的抗骨关节炎作用。在一项研究中，番红O染色和免疫组织化学分析显示，用30% FEPY治疗可显著增加DMM组的软骨降解和II型胶原蛋白表达。这些发现共同表明，30% FEPY是缓解OA进展和开发新治疗药物的有前途的候选物。
