Nanney D L, Chow M, Wozencraft B
J Exp Zool. 1975 Jul;193(1):1-14. doi: 10.1002/jez.1401930102.
Homopolar doublets of syngen 1, T. pyriformis, may be induced by treatment of conjugating pairs with immobilizing antiserum. These doublets have geometric properties and basal body populations generally indicative of separate autonomous integrative systems in the two halves. The duplex system, though metastable, is transformed through a process of "simplification" back to the simplex state. The transformation is not a single event, but a series involving changes at different times for different structures and processes; for the micronuclei and the macronuclei; for the capacity to generate two oral apparatuses through stomatogenesis and the capacity to develop them through oral replacement; for the structures at the anterior and posterior ends of the cell; for the numbers of ciliary rows and for the numbers of basal bodies which make up the rows. Although the two semicells composing a doublet are in important respects independent of each other, they are coordinated in significant ways. The positions of the contractile vacuole pores and their numbers depend not only on the number of ciliary rows in a semicell, but also on the number of ciliary rows in the opposing twin. Most notably, the probability for dual stomatogenesis, and hence the perpetuation of the doubled oral apparatus, depends on the symmetry of the semicells. The cell as a whole is maintained as an integrated unit over a prolonged interval as various aspects of duplex structure and function are progressively consolidated.
用固定抗血清处理梨形四膜虫接合对，可诱导出同基因1的同极双联体。这些双联体具有几何特性和基体群体，通常表明两半部分存在独立的自主整合系统。双联体系统虽然是亚稳态的，但会通过“简化”过程转变回单联体状态。这种转变不是一个单一事件，而是一系列事件，涉及不同结构和过程在不同时间的变化；对于微核和大核而言；对于通过口器发生产生两个口器的能力以及通过口器替换发育口器的能力而言；对于细胞前端和后端的结构而言；对于纤毛排的数量以及构成这些排的基体数量而言。尽管组成双联体的两个半细胞在重要方面相互独立，但它们在重要方面也相互协调。收缩泡孔的位置及其数量不仅取决于一个半细胞中的纤毛排数量，还取决于相对的另一半细胞中的纤毛排数量。最值得注意的是，双口器发生的概率，进而双倍口器的延续，取决于半细胞的对称性。随着双联体结构和功能的各个方面逐渐巩固，整个细胞在较长时间内保持为一个整合单元。
