Ngai Yuen Tung, Lau Darren, Mittal Parul, Hoffmann Peter
UniSA Clinical and Health Sciences, Health and Biomedical Innovation University of South Australia Adelaide South Australia Australia.
Anal Sci Adv. 2025 May 10;6(1):e70016. doi: 10.1002/ansa.70016. eCollection 2025 Jun.
Matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) is an emerging imaging tool that allows visualisation of hundreds of analytes unbiasedly in a single experiment. This paper highlights the adaptations of MALDI-MSI in different context in 2024, such as clinical diagnostic, pharmacology, forensics applications, plant metabolism and biology. Challenges and advancements were also discussed regarding sample preparation, instrumentations, data analysis, and integration of machine learning in the trend of single cell resolution and multi-omics. There are still rooms for improvements in sensitivity, spatial resolution, acquisition algorithm and data integration across multi-omics data to enable MALDI-MSI at subcellular level.
基质辅助激光解吸/电离质谱成像（MALDI-MSI）是一种新兴的成像工具，它能够在单次实验中无偏差地可视化数百种分析物。本文重点介绍了2024年MALDI-MSI在不同领域的应用，如临床诊断、药理学、法医应用、植物代谢与生物学。同时还讨论了在单细胞分辨率和多组学趋势下，MALDI-MSI在样品制备、仪器设备、数据分析以及机器学习整合方面所面临的挑战和取得的进展。在灵敏度、空间分辨率、采集算法以及跨多组学数据的数据整合方面，仍有改进空间，以实现亚细胞水平的MALDI-MSI。
