Li Songzhi, Guo Yang, Jiang Hong, Zhang Huan, Li Jiayu, Chen Yanli, Li Jie, Mao Xiangzhao, Wang Minxiao
College of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266520, PR China.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266000, PR China.
ACS Omega. 2025 Apr 22;10(17):18005-18016. doi: 10.1021/acsomega.5c01356. eCollection 2025 May 6.
Arylsulfatase catalyzes the cleavage of sulfate ester bonds and plays a role in agar desulfation, thereby enhancing agar gel strength and quality. While studying the desulfurization pathway in sp. SR43-6, a sequence encoding a potential arylsulfatase- Ars (-Ars)-was found. The enzyme, with -nitrophenyl sulfate as a substrate, exhibited optimal activity at 35 °C and pH 8.0. Its relative activity (206 U/mg) exceeded that of the recently identified arylsulfatases. Four hundred units of the enzyme removed 86.4% of sulfate groups from agar in 4 h, whereas 800 U of the enzyme removed 71.3% of sulfate groups from agar in 8 h. After enzymatic treatment, agar gel strength was enhanced by 32%, and a similar improvement was observed in agar gel strength. Enzymatic agar desulfurization offers mild, quality-retaining, and environmentally friendly advantages, augmenting industrial application prospects.
芳基硫酸酯酶催化硫酸酯键的裂解，在琼脂脱硫中发挥作用，从而提高琼脂凝胶强度和质量。在研究sp. SR43-6中的脱硫途径时，发现了一个编码潜在芳基硫酸酯酶-Ars（-Ars）的序列。该酶以对硝基苯硫酸酯为底物，在35℃和pH 8.0时表现出最佳活性。其相对活性（206 U/mg）超过了最近鉴定的芳基硫酸酯酶。400单位的该酶在4小时内从琼脂中去除了86.4%的硫酸基团，而800单位的该酶在8小时内从琼脂中去除了71.3%的硫酸基团。酶处理后，琼脂凝胶强度提高了32%，在琼脂凝胶强度方面也观察到了类似的改善。酶法琼脂脱硫具有温和、保留质量和环境友好的优点，增强了工业应用前景。
