Li Xiang, Zheng Zhenzhen, Zhou Yun, Yang Shenglong, Su Wang, Guo Heng, Ye Guangji, Wang Jian
Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, China.
Qinghai Provincial Key Laboratory of Potato Breeding, Qinghai University, Xining, China.
Front Plant Sci. 2025 Apr 25;16:1543380. doi: 10.3389/fpls.2025.1543380. eCollection 2025.
Potato ( L.), as an important food crop on the Qinghai-Tibet Plateau, is prone to low temperature and frost damage during the seedling stage, causing economic losses for farmers.
In this study, metabolome and transcriptome analyses were conducted on the leaves of Atlantic (cold-resistant) and KY140 (cold-sensitive) potato varieties following exposure to cold stress (CS).
After CS, 298 and 195 differentially accumulated metabolites (DAMs) were identified in Atlantic and KY140, respectively, with 124 common DAMs, including lipids, flavonoids, alkaloids, organic acids, amino acids and their derivatives, nucleotides and their derivatives, lignans and coumarins, phenolic acids, and terpenoids. A total of 6928 and 2428 differentially expressed genes(DEGs) were identified in Atlantic and KY140, respectively, with 1131 common DEGs. Joint analysis of DAMs and DEGs, "flavonoid-related metabolism," "lipid metabolism," and "amino acid metabolism" were plotted. Cinnamic acid, caffeic acid, naringenin, and γ-aminobutyric acid (GABA) might participate in the regulation of potato resistance to CS. The genes and encode enzymes responsible for the biosynthesis of cinnamic acid and GABA, respectively, suggesting their involvement in the regulation of cold resistance in potato.
Our results provided novel insights into the molecular mechanisms underlying cold resistance in potato.
马铃薯（L.）作为青藏高原上的一种重要粮食作物，在苗期易受低温和霜冻危害，给农民造成经济损失。
本研究对耐冷品种大西洋和冷敏品种KY140的马铃薯叶片在冷胁迫（CS）处理后进行了代谢组和转录组分析。
冷胁迫处理后，大西洋品种和KY140品种分别鉴定出298个和195个差异积累代谢物（DAM），其中有124个共有DAM，包括脂质、黄酮类化合物、生物碱、有机酸、氨基酸及其衍生物、核苷酸及其衍生物、木脂素和香豆素、酚酸和萜类化合物。大西洋品种和KY140品种分别鉴定出6928个和2428个差异表达基因（DEG），其中有1131个共有DEG。对DAM和DEG进行联合分析，绘制了“类黄酮相关代谢”“脂质代谢”和“氨基酸代谢”图。肉桂酸、咖啡酸、柚皮素和γ-氨基丁酸（GABA）可能参与马铃薯对冷胁迫抗性的调控。基因 和 分别编码负责肉桂酸和GABA生物合成的酶，表明它们参与马铃薯抗冷性的调控。
我们的结果为马铃薯抗冷性的分子机制提供了新的见解。
