Iqbal Owais, Yang Xingrun, Wang Ruoping, Wang Chun, Li Dandan, Wen Jiancheng, Ding Jiasheng, Jibril Sauban Musa, Li Chengyun, Wang Yi
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan, China.
Yunnan-CABI Joint Laboratory for Integrated Prevention and Control of Transboundary Pests, Yunnan Agricultural University, Kunming, Yunnan, China.
BMC Plant Biol. 2025 Jul 2;25(1):836. doi: 10.1186/s12870-025-06856-5.
Magnaporthe oryzae is one of the most devastating pathogens of rice, causing significant economic losses worldwide. Despite extensive studies on the M. oryzae-rice interaction, particularly focusing on the underlying resistance mechanisms, the molecular basis of rice resistance remains poorly understood.
This study employed an integrated metabolomic, transcriptomic, and genomic approach to compare the response of Diantun susceptible (D502) and resistant (D506) rice lines to M. oryzae infection at 48 h post-inoculation. A total of 588 and 595 differentially accumulated metabolites (DAMs) were identified in D502 and D506, respectively. Notably, 55% of these metabolites exhibited similar expression patterns across both lines, while 9 DAMs displayed contrasting patterns at 48 h in response to pathogen infection. Pathway analysis revealed significant regulation of flavonoid, nucleotide derivatives, phenylpropanoid and polyketide, and vitamin biosynthesis pathways, with specific metabolites from these pathways potentially contributing to resistance in D506. KEGG enrichment analysis further identified key pathways in D506, including linoleic acid metabolism, plant hormone signal transduction, α-linolenic acid metabolism, and the pentose phosphate pathway. Network analysis based on DAMs and differentially expressed genes (DEGs) highlighted eight up-regulated metabolites and their key genes responsible for resistance, which are associated with flavonoid, tryptophan, and phytohormone, resulting in suppressed M. oryzae infection in D506. The content of sakuranetin was significantly higher, and the peak in expression of their key gene OMT-9 after M. oryzae infection at 48 h, lead to an increase in phytoalexin production in the D506 line. Subsequently, exonic non-synonymous single nucleotide polymorphisms (nsSNPs) within abscisic acid synthesis (NCED1) gene, identified through genome-wide analysis, were associated with amino acid substitutions potentially affecting protein function. This finding suggests that the ABA and their key genes are essential for the resistance in D506 against M. oryzae.
In last, we conclude that our findings underscore the power of integrating metabolomics, transcriptomics, and genomics to identify key metabolites and genes underlying resistance to M. oryzae. The insights gained from this study offer valuable resources for enhancing rice breeding strategies and improving disease management in agriculture.
稻瘟病菌是水稻最具毁灭性的病原菌之一，在全球范围内造成重大经济损失。尽管对稻瘟病菌与水稻的相互作用进行了广泛研究，特别是关注潜在的抗性机制，但水稻抗性的分子基础仍知之甚少。
本研究采用综合代谢组学、转录组学和基因组学方法，比较了滇屯易感（D502）和抗性（D506）水稻品系在接种后48小时对稻瘟病菌感染的反应。在D502和D506中分别鉴定出588和595种差异积累代谢物（DAM）。值得注意的是，这些代谢物中有55%在两个品系中表现出相似的表达模式，而9种DAM在48小时时对病原体感染表现出相反的模式。通路分析显示黄酮类、核苷酸衍生物、苯丙烷类和聚酮类以及维生素生物合成通路受到显著调控，这些通路中的特定代谢物可能有助于D506的抗性。KEGG富集分析进一步确定了D506中的关键通路，包括亚油酸代谢、植物激素信号转导、α-亚麻酸代谢和磷酸戊糖途径。基于DAM和差异表达基因（DEG）的网络分析突出了八种上调的代谢物及其负责抗性的关键基因，它们与黄酮类、色氨酸和植物激素相关，导致D506中稻瘟病菌感染受到抑制。樱花素的含量显著更高，其关键基因OMT-9在接种稻瘟病菌48小时后的表达峰值导致D506品系中植保素产量增加。随后，通过全基因组分析确定的脱落酸合成（NCED1）基因内的外显子非同义单核苷酸多态性（nsSNP）与可能影响蛋白质功能的氨基酸替换有关。这一发现表明脱落酸及其关键基因对D506对稻瘟病菌的抗性至关重要。
最后，我们得出结论，我们的研究结果强调了整合代谢组学、转录组学和基因组学以鉴定稻瘟病菌抗性潜在关键代谢物和基因的作用。本研究获得的见解为加强水稻育种策略和改善农业病害管理提供了宝贵资源。
