Nazwar Tommy Alfandy, Ridwan Muhammad, Wardhana Donny Wisnu, Bal'afif Farhad, Bal'afif Fachriy, Solimun Solimun, Panjaitan Christin
Division of Neurosurgery, Department of Surgery, Brawijaya University and Saiful Anwar General Hospital, Malang, East Java, Indonesia.
Department of Surgery, Brawijaya University and Saiful Anwar General Hospital, Malang, East Java, Indonesia.
Korean J Neurotrauma. 2025 Apr 23;21(2):102-113. doi: 10.13004/kjnt.2025.21.e12. eCollection 2025 Apr.
To evaluate the effects of high-impact loads in a weight drop (WD) model of traumatic brain injury (TBI) on sustained neurobehavioral dysfunction over a 14-day period.
The experimental treatment involved craniotomy of rats, exposing the brain to a load applied via the WD method. Loads of 10 g were dropped from heights of 5, 10, and 15 cm. Neurobehavioral assessments included the Barnes maze for cognitive and affective function evaluation and the beam walking test for motor function assessment. Statistical analyses were conducted using the Statistical Package for the Social Sciences (SPSS) version 22 and GraphPad Prism version 9 software. Statistical significance was set at <0.05.
In the Barnes maze test, an increase in WD height was directly proportional to the post-test mean score, indicating poorer cognitive performance, with WD 15 cm yielding the highest mean score (146.6±16.8), followed by WD 10 cm (114.7±22.7) and WD 5 cm (100.6±18.6). Conversely, in the beam walk test, an increase in WD height contributed to a decline in motor performance, with the lowest mean score observed at WD 15 cm (20.6±9.84), while WD 10 cm (52.5±9.79) and WD 5 cm (74.7±12.5) showed less impairment.
Load-height variations in WD TBI models significantly influence neurobehavioral outcomes, with the 15 cm height having the most pronounced effect, inducing impairments in motor, cognitive, and affective functions.
评估创伤性脑损伤（TBI）重量下降（WD）模型中的高冲击负荷对14天内持续神经行为功能障碍的影响。
实验性治疗包括对大鼠进行开颅手术，通过WD方法使大脑承受负荷。将10克的重物从5厘米、10厘米和15厘米的高度落下。神经行为评估包括用于认知和情感功能评估的巴恩斯迷宫以及用于运动功能评估的横梁行走测试。使用社会科学统计软件包（SPSS）22版和GraphPad Prism 9版软件进行统计分析。统计学显著性设定为<0.05。
在巴恩斯迷宫测试中，WD高度的增加与测试后平均得分直接相关，表明认知表现较差，WD 15厘米时平均得分最高（146.6±16.8），其次是WD 10厘米（114.7±22.7）和WD 5厘米（100.6±18.6）。相反，在横梁行走测试中，WD高度的增加导致运动表现下降，WD 15厘米时平均得分最低（20.6±9.84），而WD 10厘米（52.5±9.79）和WD 5厘米（74.7±12.5）的损伤较小。
WD TBI模型中的负荷高度变化显著影响神经行为结果，15厘米高度的影响最为明显，会导致运动、认知和情感功能受损。
