Ismailova A M, Severyukhina M S, Shaykhutdinova E R, Perepechenova N A, Starkov V G, Dyachenko I A, Tsetlin V I, Utkin Yu N
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia.
Dokl Biochem Biophys. 2025 May 11. doi: 10.1134/S1607672925600137.
Heterodimeric phospholipase A2 HDP-1 from the venom of Nikolsky viper consists of two noncovalently bound subunits-HDP-1P and HDP-1In, the first of which exhibits lipolytic activity, while the second does not. The subunits have similar molecular masses and amino acid sequences. Administration of HDP-1 and HDP-1P to anesthetized rats is accompanied by prolonged hypotensive effects, the intensity of which depends on the dose. After the administration of HDP-1P, the effect develops more slowly than in the case of HDP-1, containing both subunits, while individual HDP-1In does not affect hemodynamic parameters. It was found using electrocardiography that HDP-1 and HDP-1P affect the functioning of the rat heart, causing arrhythmia, which may indicate their ability to affect the innervation of the heart. The administration of HDP-1 and its subunits also leads to changes in blood biochemical parameters, indicating their damaging effect on cells and internal organs.
来自尼氏蝰蛇毒液的异二聚体磷脂酶A2（HDP-1）由两个非共价结合的亚基组成——HDP-1P和HDP-1In，其中第一个亚基具有脂解活性，而第二个亚基没有。这些亚基具有相似的分子量和氨基酸序列。给麻醉大鼠注射HDP-1和HDP-1P会伴随长时间的降压作用，其强度取决于剂量。注射HDP-1P后，这种作用的发展比注射含有两个亚基的HDP-1时更慢，而单独的HDP-1In不影响血流动力学参数。通过心电图发现，HDP-1和HDP-1P会影响大鼠心脏的功能，导致心律失常，这可能表明它们有影响心脏神经支配的能力。注射HDP-1及其亚基还会导致血液生化参数的变化，表明它们对细胞和内脏器官有损害作用。
