Cilekar Murat, Kargin Solmaz Fatma Ozlem, Isite Nilay
Department of General Surgery, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar, Turkey.
Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Afyonkarahisar Health Sciences University, Afyonkarahisar, Turkey.
Medicine (Baltimore). 2025 May 9;104(19):e42378. doi: 10.1097/MD.0000000000042378.
Obesity is a multifactorial disease, commonly observed both worldwide and in our country, triggered by environmental and genetic factors, adversely affecting all physiological functions of the body, and leading to an increase in body fat mass. Although various variants associated with susceptibility to obesity have been identified in genomic studies, these variants explain only a small portion of the genetic basis of obesity. This case-control study investigates, for the first time in the Turkish population, the relationship between CYP1B1 gene rs1056827 and rs1056836 polymorphisms in obesity patients undergoing surgical intervention (bariatric surgery). Genotyping of the polymorphisms was performed using Real-Time PCR in 63 female and 29 male obesity patients who underwent bariatric surgery and 40 female and 51 male nonobese individuals. In our study, genotype distributions for the CYP1B1 gene rs1056836 polymorphism were found to be 51.1% CC, 40.2% CG, and 8.7% GG in the case group and 46.2% CC, 47.3% CG, and 6.6% GG in the control group. The frequency of the C allele was 71.2%, and the G allele was 28.8% in the case group, while the frequency of the C allele was 70.3%, and the G allele was 29.7% in the control group. For the rs1056827 polymorphism, the genotype distributions were 10.8% GG, 35.9% GT, and 53.3% TT in the case group and 7.7% GG, 49.4% GT, and 42.9% TT in the control group. The frequency of the G allele was 28.8%, and the T allele was 71.2% in the case group, whereas the frequency of the G allele was 32.4%, and the T allele was 67.6% in the control group. No significant difference was found between the case and control groups in terms of anthropometric measurements and biochemical parameter values for the rs1056836 and rs1056827 polymorphisms of the CYP1B1 gene. Our study is valuable as it is the first to investigate the association of CYP1B12 (rs1056827) and CYP1B13 (rs1056836) polymorphisms with obesity, and it was determined that there was no difference in the investigated polymorphisms between the control group and the obesity group.
肥胖是一种多因素疾病，在全球和我国都普遍存在，由环境和遗传因素引发，对身体的所有生理功能产生不利影响，并导致体脂量增加。尽管在基因组研究中已鉴定出与肥胖易感性相关的各种变异，但这些变异仅解释了肥胖遗传基础的一小部分。本病例对照研究首次在土耳其人群中调查了接受手术干预（减肥手术）的肥胖患者中CYP1B1基因rs1056827和rs1056836多态性之间的关系。采用实时荧光定量PCR对63例接受减肥手术的肥胖女性和29例肥胖男性患者以及40例非肥胖女性和51例非肥胖男性个体进行多态性基因分型。在我们的研究中，病例组中CYP1B1基因rs1056836多态性的基因型分布为CC占51.1%、CG占40.2%、GG占8.7%，对照组中CC占46.2%、CG占47.3%、GG占6.6%。病例组中C等位基因频率为71.2%，G等位基因频率为28.8%，对照组中C等位基因频率为70.3%，G等位基因频率为29.7%。对于rs1056827多态性，病例组的基因型分布为GG占10.8%、GT占35.9%、TT占53.3%，对照组中GG占7.7%、GT占49.4%、TT占42.9%。病例组中G等位基因频率为28.8%，T等位基因频率为71.2%，对照组中G等位基因频率为32.4%，T等位基因频率为67.6%。在CYP1B1基因的rs1056836和rs1056827多态性方面，病例组和对照组在人体测量指标和生化参数值上未发现显著差异。我们的研究具有重要价值，因为它首次调查了CYP1B12（rs1056827）和CYP1B13（rs1056836）多态性与肥胖的关联，并且确定在对照组和肥胖组之间所研究的多态性没有差异。
