与亲代DA大鼠相比，坐骨神经损伤和修复后，DA基因同源导入大鼠显示出基因表达增加和雪旺细胞凋亡，但神经再生未受影响。

DA.-congenic rats display increased gene expression and Schwann cell apoptosis but unaffected nerve regeneration compared to parental DA rats after sciatic nerve injury and repair.

作者信息

Stenberg Lena, Jewett Michael, Dueñas Rey Alfredo, Swanberg Maria, Dahlin Lars B

机构信息

Department of Translational Medicine - Hand Surgery, Lund University, Malmö, Sweden.

Department of Experimental Medicine, Lund University, Lund, Sweden.

出版信息

Front Cell Dev Biol. 2025 Apr 28;13:1536347. doi: 10.3389/fcell.2025.1536347. eCollection 2025.

DOI:10.3389/fcell.2025.1536347

PMID:40356597

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12066652/

Abstract

INTRODUCTION

The rat locus, containing (), regulates the degeneration of central nervous system (CNS) neurons in toxin-, protein-, and injury-based models. We hypothesize that Piebald Virol Glaxo.1AV1 (PVG) alleles in confer protection and increased axonal outgrowth after peripheral nerve injury and repair.

METHODS

DA rats (n = 14) and DA rats with PVG alleles in the locus (DA. n = 14) were subjected to sciatic nerve transection and immediate repair. After 6 days, axonal outgrowth and protein and gene expression were analyzed in injured and uninjured nerves and dorsal root ganglia (DRG).

RESULTS

No differences in axonal outgrowth were observed between strains, but the number of apoptotic Schwann cells in the injured distal nerve end was higher in DA. than in DA rats (p = 0.003). In both strains, gene- and protein expression of activating transcription factor 3 (ATF3) and 27-kDa heat shock protein (HSP27, i.e., Hspb1) were increased in injured vs. uninjured DRG. In DA. rats, gene expression was lower in injured vs. uninjured DRG (p = 0.043) but higher than in DA rats in injured nerves (p = 0.008) and injured DRG (p = 0.008). DA. had higher gene expression of (p = 0.016) and (p = 0.032) in injured nerves than DA rats.

DISCUSSION

Results highlight the complexity of nerve injury and repair, supporting further investigation of in nerve regeneration.

摘要

引言

大鼠的基因座包含（），在基于毒素、蛋白质和损伤的模型中调节中枢神经系统（CNS）神经元的退化。我们假设该基因座中的花斑病毒格拉克索1AV1（PVG）等位基因在周围神经损伤和修复后具有保护作用并能促进轴突生长。

方法

将14只DA大鼠和14只该基因座带有PVG等位基因的DA大鼠（DA.）进行坐骨神经横断并立即修复。6天后，对损伤和未损伤的神经以及背根神经节（DRG）进行轴突生长、蛋白质和基因表达分析。

结果

两品系间轴突生长未见差异，但DA.大鼠损伤远端神经末梢中凋亡雪旺细胞的数量高于DA大鼠（p = 0.003）。在两个品系中，损伤的DRG与未损伤的DRG相比，激活转录因子3（ATF3）和27 kDa热休克蛋白（HSP27，即Hspb1）的基因和蛋白表达均增加。在DA.大鼠中，损伤的DRG与未损伤的DRG相比，基因表达较低（p = 0.043），但在损伤神经（p = 0.008）和损伤DRG（p = 0.008）中高于DA大鼠。DA.大鼠损伤神经中（p = 0.016）和（p = 0.032）的基因表达高于DA大鼠。