Dalbro Solveig E J, Elsais Ahmed, Rydning Siri Lynne, Toft Mathias, Kerty Emilia, Larsen Stig E
Department of Neurology, Oslo University Hospital, Oslo, Norway.
Institute of Clinical Medicine, University of Oslo, Oslo, Norway.
Front Neurol. 2025 Apr 28;16:1556314. doi: 10.3389/fneur.2025.1556314. eCollection 2025.
Eye movements have been proposed as biomarkers to track disease progression and treatment effects in neurological diseases. Before such variables are used in the clinic or in drug trials, properties such as measurement error must be documented. In this study, we assessed repeatability, reliability, and stability of fixation, smooth pursuit, and saccade measurements in patients with Parkinson's disease, cerebellar ataxia, and healthy adults.
Fixation, smooth pursuit, and saccade metrics were measured in 16 patients with Parkinson's disease, 16 patients with ataxia, and 25 healthy adults with an eye tracker (BulbiCam). The same operator repeated the measurements six times over 2 days in the patient group and two times the same day in the healthy adults. Reliability, repeatability, and stability were assessed with the intraclass correlation coefficient (ICC), Bland-Altman plots with the Agreement Index, and the Stability Index, respectively.
Mean pupil size in the fixation test and latency, accuracy and peak velocity in the pro-saccade test were found reliable, repeatable, and stable. Mean and max fixation in the fixation test were found reliable and stable. Smooth pursuit measurements were found repeatable within patients and stable, but not reliable.
The saccade and pupil variables may be used both on a population level and for individual patient follow-up. Mean and max fixation duration may be used on the population level but used in the clinical evaluation on individual patients they need to be repeated.
眼球运动已被提议作为追踪神经疾病进展和治疗效果的生物标志物。在这些变量用于临床或药物试验之前，必须记录诸如测量误差等特性。在本研究中，我们评估了帕金森病、小脑共济失调患者以及健康成年人的注视、平稳跟踪和扫视测量的可重复性、可靠性和稳定性。
使用眼动仪（BulbiCam）对16例帕金森病患者、16例共济失调患者和25名健康成年人进行注视、平稳跟踪和扫视指标测量。在患者组中，同一名操作人员在2天内重复测量6次，在健康成年人中则在同一天重复测量2次。分别使用组内相关系数（ICC）、带有一致性指数的布兰德-奥特曼图以及稳定性指数评估可靠性、可重复性和稳定性。
发现注视测试中的平均瞳孔大小以及前向扫视测试中的潜伏期、准确性和峰值速度可靠、可重复且稳定。发现注视测试中的平均注视和最大注视可靠且稳定。发现平稳跟踪测量在患者内部可重复且稳定，但不可靠。
扫视和瞳孔变量可用于群体水平以及个体患者随访。平均和最大注视持续时间可用于群体水平，但在个体患者的临床评估中使用时需要重复测量。
