Najari Parisa, Akbarzadeh Sama, Rajabi Ali, Tayefeh-Gholami Samaneh, Abbaslou Elaheh Malek, Ghasemzadeh Tooraj, Hosseinpourfeizi Mohammadali, Safaralizadeh Reza
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Department of Biophysics, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.
Genes Cancer. 2025 May 9;16:18-25. doi: 10.18632/genesandcancer.241. eCollection 2025.
Gastric cancer (GC) is a multifactorial disease with a high death rate due to the unknown mechanisms involved in the developing, progressing, and late diagnosing GC. Several cancers have been linked to Long non-coding RNAs (lncRNAs), including GC, through differential expression. They play a crucial role in tumorigenesis pathways as modulatory factors, making them intriguing clinical and diagnostic biomarkers for many malignancies. This study's objective is to compare the lncRNAs and expression levels in tumoral tissues to marginal tissues and the clinicopathological features of patients.
100 GC patients' tumoral and marginal tissue samples from Tabriz's Valiasr Hospital were gathered for this case-control research. To determine the expression level of and lncRNAs in GC, total RNA was extracted, and the qRT-PCR technique was employed. Compared to adjacent marginal tissues, the tumor tissue of patients with GC showed a significant increase in the expression levels of and ( < 0.0001). The expression ratio of lncRNA and did not significantly correlate with clinicopathological variables. The ROC curve's findings lead to the conclusion that the genes lncRNAs and , with AUC values of 0.68 and 0.79, respectively, suggest that they could play carcinogenic roles in GC and may act as moderate diagnostic biomarkers for GC.
In GC, and may be utilized as therapeutic targets and prognostic biomarkers, respectively.
胃癌（GC）是一种多因素疾病，由于其发生、发展和晚期诊断涉及的机制不明，死亡率很高。包括胃癌在内的几种癌症已通过差异表达与长链非编码RNA（lncRNAs）相关联。它们作为调节因子在肿瘤发生途径中起关键作用，使其成为许多恶性肿瘤引人关注的临床和诊断生物标志物。本研究的目的是比较肿瘤组织与边缘组织中lncRNAs的表达水平以及患者的临床病理特征。
本病例对照研究收集了大不里士瓦利阿斯尔医院100例例胃癌患者的肿瘤组织和边缘组织样本。为了测定GC中lncRNAs的表达水平，提取了总RNA，并采用qRT-PCR技术。与相邻边缘组织相比，GC患者的肿瘤组织中lncRNAs的表达水平显著升高（<0.0001）。lncRNA的表达率与临床病理变量无显著相关性。ROC曲线的结果得出结论，lncRNAs基因的AUC值分别为0.68和0.79，表明它们可能在GC中发挥致癌作用，并可能作为GC的中度诊断生物标志物。
在GC中，lncRNAs可能分别用作治疗靶点和预后生物标志物。
