Liu Xin, Peng Yixiu, Wu Jun, Qian Hongwu, Lv Xiaoqian, Li Fan, Jin Kai, Niu Yingjie, Song Jiuzhou, Han Wei, Chen Guohong, Li Bichun, Zuo Qisheng
Key Laboratory of Animal Genetics, Breeding and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
Animal & Avian Sciences, University of Maryland, College Park, MA 20742, USA.
Poult Sci. 2025 Jul;104(7):105259. doi: 10.1016/j.psj.2025.105259. Epub 2025 May 3.
In the field of genetic breeding, primordial germ cells (PGCs) have become essential cells for gene editing and genetic improvement due to their unique developmental potential and genetic characteristics. However, the low proliferation efficiency and instability of culture systems pose significant challenges, severely limiting the application of PGCs in genetic breeding. In this study, the biological effects of ovotransferrin on chicken PGCs, focusing on its role in regulating key cellular processes. Treatment with 0.5 mg/ml ovotransferrin significantly promoted cell proliferation, enhanced cell adhesion, reduced oxidative stress, and suppressed apoptosis in cultured PGCs. These findings reveal a regulatory role of ovotransferrin in maintaining PGCs survival and function, providing new insights into optimizing PGC culture systems through targeted modulation of cell fate.
在遗传育种领域，原始生殖细胞（PGCs）因其独特的发育潜能和遗传特性，已成为基因编辑和遗传改良的关键细胞。然而，其增殖效率低以及培养系统的不稳定性带来了重大挑战，严重限制了PGCs在遗传育种中的应用。在本研究中，重点关注卵转铁蛋白对鸡PGCs关键细胞过程的调控作用，研究其生物学效应。用0.5mg/ml卵转铁蛋白处理可显著促进培养的PGCs细胞增殖、增强细胞黏附、降低氧化应激并抑制细胞凋亡。这些发现揭示了卵转铁蛋白在维持PGCs存活和功能方面的调节作用，为通过靶向调控细胞命运优化PGCs培养系统提供了新的见解。
