Peroxisomal Sulfite Oxidase (SOX), an alternative source of NO in higher plants which is upregulated by HS.

Nitric oxide (NO) is a free radical that is endogenously produced in plant cells, though its enzymatic synthesis remains a subject of ongoing debate. Plant peroxisomes, subcellular compartments with active nitro-oxidative metabolism, play a role in various metabolic pathways. Sulfite oxidase (SOX), a peroxisomal enzyme requiring the molybdenum cofactor (MoCo), catalyzes the oxidation of sulfite (SO) to sulfate (SO), along with the concomitant production of HO. Using reconstituted recombinant SOX from pepper (Capsicum annuum L.) fruit, it was shown that this enzyme has the capacity to generate NO using nitrite (NO) as a substrate and NADH as an electron donor which was detected by electron paramagnetic resonance (EPR) spectroscopy coupled with the spin-trapping method. Furthermore, this NO generation was upregulated in the presence of hydrogen sulfide (HS) but was downregulated by HO which highlights the relationship between HO, NO, and HS. This data opens new avenues for understanding the enzymatic sources of NO in higher plants, particularly within peroxisomes.