Yin Ru, Chang Mingdong, Ma Rui, Wang Jianqiao, Wang Nana, Xiao Tangfu, Hirai Hirofumi
Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China.
Faculty of Global Interdisciplinary Science and Innovation, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
J Agric Food Chem. 2025 May 28;73(21):12877-12886. doi: 10.1021/acs.jafc.5c01521. Epub 2025 May 13.
Imidaclothiz (IMZ), an innovative neonicotinoid insecticide, has attracted significant interest due to its environmental persistence and consequent ecological implications. In this research, the white-rot fungus YK-624 was used to degrade IMZ, unveiling a novel fungal degradation mechanism. The results demonstrated that IMZ was efficiently degraded by YK-624. Transcriptomic analysis revealed that IMZ-induced stress triggered a cascade of enzymatic and cellular defense responses that are instrumental in facilitating its biodegradation. Through inhibitor experiments and enzyme activity profiling, cytochrome P450 and manganese peroxidase (MnP) were identified to play crucial roles in IMZ biodegradation. Additionally, three metabolites were isolated and identified by NMR, and two innovative degradation pathways involving hydroxylation and nitro reduction were proposed. Toxicity assessment suggested the reduced environmental risk of IMZ after its degradation by YK-624. These findings provided insights into the IMZ degradation mechanism and highlighted the potential of white-rot fungi in neonicotinoid bioremediation.
吡虫啉（IMZ）是一种新型烟碱类杀虫剂，因其环境持久性及其带来的生态影响而备受关注。在本研究中，白腐真菌YK - 624被用于降解IMZ，揭示了一种新的真菌降解机制。结果表明，YK - 624能有效降解IMZ。转录组分析显示，IMZ诱导的应激引发了一系列酶促和细胞防御反应，这些反应有助于其生物降解。通过抑制剂实验和酶活性分析，确定细胞色素P450和锰过氧化物酶（MnP）在IMZ生物降解中起关键作用。此外，通过核磁共振分离并鉴定了三种代谢产物，并提出了两条涉及羟基化和硝基还原的创新降解途径。毒性评估表明，IMZ经YK - 624降解后环境风险降低。这些发现为IMZ降解机制提供了见解，并突出了白腐真菌在新烟碱类生物修复中的潜力。
