Wang Beijia, Wang Jianqiao, Yin Ru, Zhang Xue, Zeng Zhonghua, Zhang Ge, Wang Nana, Hirai Hirofumi, Xiao Tangfu
Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, School of Environmental Science and Engineering, Guangzhou University, Guangzhou, 510006 China.
Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529 Japan.
3 Biotech. 2022 Sep;12(9):225. doi: 10.1007/s13205-022-03298-w. Epub 2022 Aug 13.
Bisphenol A (BPA) is a representative example of an endocrine-disrupting chemical. It is one of the most produced chemical substances in the world, but it causes harmful effects in organisms, such that the effective degradation of BPA is critical. The white-rot fungus YK-624 has been shown to effectively degrade BPA under ligninolytic and non-ligninolytic conditions. However, it is still unclear what kinds of enzymes are involved in BPA degradation. To explore the mechanism of BPA degradation, the present study analysed the functional genes of YK-624 using RNA-sequencing (RNA-Seq). Oxidation-reduction process and metabolic pathway were enriched under ligninolytic and non-ligninolytic conditions by Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. It is suggested that BPA might be used as a carbon source by YK-624. Lignin peroxidase and cytochrome P450 were detected in upregulated differentially expressed genes (DEGs). The lignin-degrading enzyme lignin peroxidase and the intracellular cytochrome P450 system were involved in BPA degradation by YK-624, respectively. Furthermore, quantitative real-time PCR (qPCR) was used to validate the reliability of the RNA-Seq results.
The online version contains supplementary material available at 10.1007/s13205-022-03298-w.
双酚A（BPA）是一种典型的内分泌干扰化学物质。它是世界上产量最高的化学物质之一，但会对生物体产生有害影响，因此有效降解双酚A至关重要。白腐真菌YK - 624已被证明在木质素分解和非木质素分解条件下都能有效降解双酚A。然而，目前仍不清楚哪些酶参与了双酚A的降解过程。为了探究双酚A的降解机制，本研究利用RNA测序（RNA - Seq）分析了YK - 624的功能基因。通过基因本体论（GO）分析和京都基因与基因组百科全书（KEGG）通路分析，发现在木质素分解和非木质素分解条件下，氧化还原过程和代谢途径均得到了富集。这表明YK - 624可能将双酚A用作碳源。在上调的差异表达基因（DEG）中检测到了木质素过氧化物酶和细胞色素P450。木质素降解酶木质素过氧化物酶和细胞内细胞色素P450系统分别参与了YK - 624对双酚A的降解过程。此外，还使用了定量实时PCR（qPCR）来验证RNA - Seq结果的可靠性。
在线版本包含可在10.1007/s13205 - 022 - 03298 - w获取的补充材料。
