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响应面优化揭示了银杏叶中每月总黄酮峰值及其相应的DPPH清除活性。

Response surface optimization reveals monthly total flavonoid peaks in Ginkgo biloba leaves with corresponding DPPH scavenging activity.

作者信息

Shen Xiaoyu, Lu Yangjie, Zhu Qi, Zhang Yangbo, Zeng Li

机构信息

School of Pharmacy, Hunan Engineering Research Center of Development and Utilization of Traditional Chinese Medicine in Southwest Hunan, Shaoyang University, Shaoyang, 422000, China.

出版信息

Sci Rep. 2025 May 13;15(1):16613. doi: 10.1038/s41598-025-01635-6.

DOI:10.1038/s41598-025-01635-6
PMID:40360604
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12075695/
Abstract

The study investigated the influence of ethanol concentration, reflux time, and reflux temperature on the content of total flavonoids (TFs) in Ginkgo biloba (GB) leaves. The optimal extraction conditions were determined using the response surface (RS) methodology. The fluctuation of flavonoid content in ethanol extracts of GB leaves across different months was compared with these optimized extraction parameters. Additionally, we investigated the antioxidant activity against DPPH free radicals for the GB extract in the month with the highest flavonoid content. Following RS optimization, the optimal extraction conditions for GB flavonoids were identified as follows: extraction rate of 1.175% at 61 °C for 30 min using 77% ethanol. Under these optimized conditions, the total flavonoid content of GB exhibited a bi-peak pattern, peaking in May and August. The extract obtained in May showed superior DPPH free radical-scavenging ability. These findings provide valuable insights for selecting raw materials for future preparations of GB products.

摘要

该研究调查了乙醇浓度、回流时间和回流温度对银杏叶中总黄酮（TFs）含量的影响。采用响应面（RS）方法确定了最佳提取条件。将银杏叶乙醇提取物中黄酮含量在不同月份的波动情况与这些优化的提取参数进行了比较。此外，我们研究了黄酮含量最高月份的银杏提取物对DPPH自由基的抗氧化活性。经过RS优化，确定银杏黄酮的最佳提取条件如下：使用77%乙醇在61℃下提取30分钟，提取率为1.175%。在这些优化条件下，银杏叶的总黄酮含量呈现双峰模式，在5月和8月达到峰值。5月获得的提取物表现出更强的DPPH自由基清除能力。这些发现为未来银杏产品制备的原料选择提供了有价值的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a962/12075695/4cea309048cd/41598_2025_1635_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a962/12075695/79af8ba44f2d/41598_2025_1635_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a962/12075695/0bc80091fa27/41598_2025_1635_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a962/12075695/eed736b1db76/41598_2025_1635_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a962/12075695/4cea309048cd/41598_2025_1635_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a962/12075695/79af8ba44f2d/41598_2025_1635_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a962/12075695/0bc80091fa27/41598_2025_1635_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a962/12075695/eed736b1db76/41598_2025_1635_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a962/12075695/4cea309048cd/41598_2025_1635_Fig4_HTML.jpg

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