Wang Ronghui, Liu Zhenhua, Yan Jipeng, Hua Siqi, Wang Zhiqian, Yan Zixun, Xie Xue, Hao Junnian, Zhou Dan, Zhou Jianqiao, Zhao Weili, Zheng Yuanyi, Tang Mengxing, Zhan Weiwei
Department of Ultrasound, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, People's Republic of China.
Ultrasound Lab for Imaging and Sensing, Department of Bioengineering, Imperial College London, London, SW7 2AZ, UK.
Sci Rep. 2025 May 13;15(1):16533. doi: 10.1038/s41598-025-01615-w.
To investigate the ability of super-resolution microcirculation imaging in identifying high-risk histologic transformation (HT) regions, directing the targeted biopsies, and promising timely chemotherapy escalation and outcome improvement. Initially, a retrospective analysis (January 1, 2018-January 1, 2022) of indolent follicular lymphoma (FL) or aggressive diffuse large B cell lymphoma was conducted to identify imaging-based markers of distinguishing aggressive lymphoma from indolent lymphoma. Subsequently, the prospective research consecutively enrolled histologically confirmed FL patients between February 1, 2022, and May 31, 2024, to validate the diagnostic performance of these investigated indicators in differentiating aggressive transformed FL from FL. Diagnostic performance and the diagnostic consistency associated with time were assessed. A total of 132 participants were enrolled: 52 (age 53 years ± 14 [SD]; 34 males) in the retrospective cohort for development and 80 (age 55 years ± 12 [SD]; 34 males), including 10 initial biopsy-confirmed HT, in the prospective cohort for validation. Super-resolution microcirculation imaging demonstrated excellent sensitivity (100%; 95% CI 69.15-100%) and specificity (97.14%; 95% CI 90.06-99.65%) for HT detection, with minimal risk of missed lesions at initial diagnosis. This technique shows potential in early HT detection, facilitating timely chemotherapy escalation and reducing the need for repeat biopsies.
为了研究超分辨率微循环成像在识别高风险组织学转化（HT）区域、指导靶向活检以及有望及时升级化疗并改善预后方面的能力。最初，对惰性滤泡性淋巴瘤（FL）或侵袭性弥漫性大B细胞淋巴瘤进行了回顾性分析（2018年1月1日至2022年1月1日），以确定区分侵袭性淋巴瘤与惰性淋巴瘤的基于影像的标志物。随后，前瞻性研究连续纳入了2022年2月1日至2024年5月31日期间经组织学确诊的FL患者，以验证这些研究指标在区分侵袭性转化型FL与FL方面的诊断性能。评估了诊断性能以及与时间相关的诊断一致性。总共招募了132名参与者：回顾性队列中有52名（年龄53岁±14[标准差]；34名男性）用于研发，前瞻性队列中有80名（年龄55岁±12[标准差]；34名男性），包括10名初始活检确诊为HT的患者用于验证。超分辨率微循环成像对HT检测显示出优异的敏感性（100%；95%置信区间69.15 - 100%）和特异性（97.14%；95%置信区间90.06 - 99.65%），在初始诊断时漏诊病变的风险极小。这项技术在早期HT检测中显示出潜力，有助于及时升级化疗并减少重复活检的需求。
