Chen Chen, Wang Fang, Chen Nanying, Dong Lina, Zhang Wei, He Ji, Zhu Faming
HLA Typing Laboratory, Blood Center of Zhejiang Province, Hangzhou, China.
Int J Immunogenet. 2025 Aug;52(4):189-194. doi: 10.1111/iji.12716. Epub 2025 May 13.
Some associations between Treponema pallidum (TP) susceptibility and human leukocyte antigen (HLA) loci at low resolution have been reported. However, the data for TP infection and HLA alleles at high resolution are limited. The purpose of this study was to perform a high-resolution screen for HLA alleles that confer susceptibility to TP infection in the Chinese Han population. A total of 184 individuals with TP infection were included in the study, and 254 unrelated healthy blood donors were included in the control group. The samples were genotyped for the HLA-A, -C, -B, -DRB1, -DQB1 and -DPB1 loci using next-generation sequencing (NGS) technology. The correlations between TP infection and the alleles and haplotypes of HLA loci were determined by statistical analysis. Five HLA alleles, including HLA-A32:01 (0.00% vs. 1.57%, p = 0.024), -B52:01 (1.36% vs. 4.13%, p = 0.025), -C01:02 (22.55% vs. 16.54%, p = 0.029), -C04:03 (1.63% vs. 0.2%, p = 0.046) and -DQB105:03 (1.63% vs. 4.13%, p = 0.046), are potentially associated with TP infection. However, no significant difference was detected after p value correction. The frequency of the HLA-A33:03-C03:02-B58:01-DRB103:01-DQB102:01-DPB105:01 haplotype in the control group was greater than that in the TP infection group (p = 0.002). In contrast, the frequency of the HLA-A02:07-C01:02-B46:01-DRB108:03-DQB106:01-DPB102:01, HLA-A11:01-C03:04-B13:01-DRB109:01-DQB103:03-DPB105:01, HLA-A11:01-C07:02-B40:01-DRB108:03-DQB106:01-DPB102:01 and HLA-A30:01-C06:02-B13:02-DRB107:01-DQB102:02-DPB102:01 haplotypes were lower in the control group than in the TP infection group (p < 0.05). HLA-C01:02 and -C04:03 may confer susceptibility to TP infection, whereas A32:01, -B52:01 and -DQB105:03 may protect against TP infection. These data are helpful in preventing and controlling TP transmission.
已经报道了梅毒螺旋体（TP）易感性与低分辨率人类白细胞抗原（HLA）基因座之间的一些关联。然而，高分辨率下TP感染与HLA等位基因的数据有限。本研究的目的是对中国汉族人群中赋予TP感染易感性的HLA等位基因进行高分辨率筛查。本研究共纳入184例TP感染个体，对照组纳入254名无关健康献血者。使用下一代测序（NGS）技术对HLA-A、-C、-B、-DRB1、-DQB1和-DPB1基因座进行基因分型。通过统计分析确定TP感染与HLA基因座的等位基因和单倍型之间的相关性。五个HLA等位基因，包括HLA-A32:01（0.00%对1.57%，p = 0.024）、-B52:01（1.36%对4.13%，p = 0.025）、-C01:02（22.55%对16.54%，p = 0.029）、-C04:03（1.63%对0.2%，p = 0.046）和-DQB105:03（1.63%对4.13%，p = 0.046），可能与TP感染相关。然而，p值校正后未检测到显著差异。对照组中HLA-A33:03-C03:02-B58:01-DRB103:01-DQB102:01-DPB105:01单倍型的频率高于TP感染组（p = 0.002）。相反，对照组中HLA-A02:07-C01:02-B46:01-DRB108:03-DQB106:01-DPB102:01、HLA-A11:01-C03:04-B13:01-DRB109:01-DQB103:03-DPB105:01、HLA-A11:01-C07:02-B40:01-DRB108:03-DQB106:01-DPB102:01和HLA-A30:01-C06:02-B13:02-DRB107:01-DQB102:02-DPB102:01单倍型的频率低于TP感染组（p < 0.05）。HLA-C01:02和-C04:03可能赋予TP感染易感性，而A32:01、-B52:01和-DQB105:03可能预防TP感染。这些数据有助于预防和控制TP传播。
