Khanuntong Sriprapai, Tupmongkol Tamonwan, Phiancharoen Sirilak, Tiyapan Apiwat, Kupatawintu Pawinee, Chaiwanichsiri Dootchai, Maiers Martin, Chiewsilp Pimol
Reference Laboratory Excellence Centre, National Blood Centre, Thai Red Cross Society, Thailand.
Reference Laboratory Excellence Centre, National Blood Centre, Thai Red Cross Society, Thailand.
Transplant Cell Ther. 2025 May 5. doi: 10.1016/j.jtct.2025.04.014.
The HLA allele and haplotype frequencies were calculated from 110,007 voluntary donors with HLA high-resolution typing and 263,542 voluntary donors with medium to high-resolution typing. This is the first study of a large samples with high-resolution HLA typing in Thai population. The frequencies of HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 alleles and haplotypes were calculated from typing by next generation sequencing (NGS). A total number of G groups for HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 were 44, 83, 38, 42, 16 and 39, respectively. A total number of 2-field single allele for HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 were 142, 196, 122, 122, 71, and 124, respectively. The variety of each HLA alleles in the Thais ranging from 1.86% to 5.58% of the total alleles in IMGT/HLA database. The frequencies of allele following common, intermediate and well-documented (CIWD) groups for HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 were 104, 155, 87, 98, 48 and 96, respectively. The alleles with frequency greater than 5% were A02:03, A11:01, A33:03, A02:07, A24:02, A24:07, B46:01, B15:02, B44:03, C01:02, C07:02, C03:04, C08:01, DRB112:02, DRB114:54 and DQB103:03. The haplotypes with frequency greater than 10% were A33:03∼C03:02∼B58:01∼DRB103:01∼DQB102:01, A02:07∼C01:02∼B46:01∼DRB109:01∼DQB103:03,A33:03∼C07:01:01G∼B44:03∼DRB107:01∼DQB102:01:01G and A11:01∼C08:01∼ B15:02∼DRB112:02∼ DQB103:01. These findings enable the registry to predict the opportunity to find good matched organ and stem cell donors for the patient with rare HLA typing.
HLA等位基因和单倍型频率是根据110,007名进行了HLA高分辨率分型的自愿捐献者以及263,542名进行了中高分辨率分型的自愿捐献者计算得出的。这是泰国人群中首次对大样本进行高分辨率HLA分型的研究。HLA-A、-B、-C、-DRB1、-DQB1和-DPB1等位基因及单倍型频率通过下一代测序（NGS）分型计算得出。HLA-A、-B、-C、-DRB1、-DQB1和-DPB1的G组总数分别为44、83、38、42、16和39。HLA-A、-B、-C、-DRB1、-DQB1和-DPB1的双字段单等位基因总数分别为142、196、122、122、71和124。泰国人中每个HLA等位基因的种类在IMGT/HLA数据库中占总等位基因的1.86%至5.58%。HLA-A、-B、-C、-DRB1、-DQB1和-DPB1的常见、中间和充分记录（CIWD）组等位基因频率分别为104、155、87、98、48和96。频率大于5%的等位基因为A02:03、A11:01、A33:03、A02:07、A24:02、A24:07、B46:01、B15:02、B44:03、C01:02、C07:02、C03:04、C08:01、DRB112:02、DRB114:54和DQB103:03。频率大于10%的单倍型为A33:03∼C03:02∼B58:01∼DRB103:01∼DQB102:01、A02:07∼C01:02∼B46:01∼DRB109:01∼DQB103:03、A33:03∼C07:01:01G∼B44:03∼DRB107:01∼DQB102:01:01G以及A11:01∼C08:01∼ B15:02∼DRB112:02∼ DQB103:01。这些发现使登记处能够预测为罕见HLA分型患者找到良好匹配器官和干细胞供体的机会。
