Allele and Haplotype Frequencies in High-Resolution Typing of Human Leukocyte Antigen -A, -B, -C, -DRB1, -DQB1 and -DPB1 in Thai Voluntary Stem Cell Donors.

The HLA allele and haplotype frequencies were calculated from 110,007 voluntary donors with HLA high-resolution typing and 263,542 voluntary donors with medium to high-resolution typing. This is the first study of a large samples with high-resolution HLA typing in Thai population. The frequencies of HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 alleles and haplotypes were calculated from typing by next generation sequencing (NGS). A total number of G groups for HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 were 44, 83, 38, 42, 16 and 39, respectively. A total number of 2-field single allele for HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 were 142, 196, 122, 122, 71, and 124, respectively. The variety of each HLA alleles in the Thais ranging from 1.86% to 5.58% of the total alleles in IMGT/HLA database. The frequencies of allele following common, intermediate and well-documented (CIWD) groups for HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 were 104, 155, 87, 98, 48 and 96, respectively. The alleles with frequency greater than 5% were A02:03, A11:01, A33:03, A02:07, A24:02, A24:07, B46:01, B15:02, B44:03, C01:02, C07:02, C03:04, C08:01, DRB112:02, DRB114:54 and DQB103:03. The haplotypes with frequency greater than 10% were A33:03∼C03:02∼B58:01∼DRB103:01∼DQB102:01, A02:07∼C01:02∼B46:01∼DRB109:01∼DQB103:03,A33:03∼C07:01:01G∼B44:03∼DRB107:01∼DQB102:01:01G and A11:01∼C08:01∼ B15:02∼DRB112:02∼ DQB103:01. These findings enable the registry to predict the opportunity to find good matched organ and stem cell donors for the patient with rare HLA typing.