Szajewski Mariusz, Ciesielski Maciej, Pęksa Rafał, Kurek Piotr, Stańczak Michał, Walczak Jakub, Zieliński Jacek, Kruszewski Wiesław Janusz
Department of Oncological Surgery, Gdynia Oncology Centre, 81-519 Gdynia, Poland.
Department of Oncological Surgery, Faculty of Health Sciences with the Institute of Maritime and Tropical Medicine, Medical University of Gdansk, 80-210 Gdansk, Poland.
Cancers (Basel). 2025 Apr 23;17(9):1406. doi: 10.3390/cancers17091406.
The objective of this study was to assess the relationship of VEGF-C and LVD with pathoclinical factors of potential prognostic value and with the survival time of gastric cancer patients.
A total of 103 radically operated patients for gastric cancer who did not undergo neoadjuvant therapy were included in this study. The minimum follow-up period after surgery was 61 months. VEGF-C and lymphatic vessels were immunohistochemically determined using antibodies, including VEGF-C (c-20) sc 1881-Goat Polyclonal IgG (Santa Cruz Biotechnology) and Podoplanin D2-40 Mouse Monoclonal Antibody (ROCHE). The relationship between VEGF-C expression in gastric adenocarcinoma cells and the density of lymphatic vessels at the periphery of the primary tumor was assessed, along with the relationships of VEGF-C and LVD with selected pathoclinical parameters of gastric cancer and prognosis.
VEGF-C overexpression was associated with increased LVD (Mann-Whitney U test, = 0.03) and the Lauren intestinal type of cancer (Pearson's chi-square test, < 0.001). Increased LVD was more often associated with cancers located beyond the cardia (Mann-Whitney U test, = 0.04). We did not demonstrate an association of VEGF-C or LVD with OS or with prognostic features, such as pT, pN, or pTNM staging. However, in the Lauren intestinal type of cancer, VEGF-C overexpression correlated with shorter OS (log-rank, = 0.01) and, at the level of = 0.05 in multivariate analysis, it had an independent negative prognostic value.
Peritumoral overexpression of VEGF-C in primary gastric cancer tumors is associated with increased LVD. The Lauren intestinal type of cancer is associated with VEGF-C overexpression. The overexpression of VEGF-C in intestinal-type gastric cancer is associated with worse prognosis.
本研究的目的是评估血管内皮生长因子C（VEGF-C）和淋巴管密度（LVD）与具有潜在预后价值的病理临床因素以及胃癌患者生存时间之间的关系。
本研究纳入了103例未接受新辅助治疗的接受胃癌根治性手术的患者。术后最短随访期为61个月。使用包括VEGF-C（c-20）sc 1881-山羊多克隆IgG（圣克鲁斯生物技术公司）和血小板内皮细胞黏附分子-1（Podoplanin）D2-40小鼠单克隆抗体（罗氏公司）在内的抗体，通过免疫组织化学方法测定VEGF-C和淋巴管。评估胃腺癌细胞中VEGF-C表达与原发肿瘤周边淋巴管密度之间的关系，以及VEGF-C和LVD与胃癌选定的病理临床参数及预后之间的关系。
VEGF-C过表达与LVD增加相关（曼-惠特尼U检验，P = 0.03），与劳伦肠型癌相关（Pearson卡方检验，P < 0.001）。LVD增加更常与贲门以外部位的癌症相关（曼-惠特尼U检验，P = 0.04）。我们未证明VEGF-C或LVD与总生存期（OS）或与预后特征（如pT、pN或pTNM分期）相关。然而，在劳伦肠型癌中，VEGF-C过表达与较短的OS相关（对数秩检验，P = 0.01），在多变量分析中，当P = 0.05时，它具有独立的负性预后价值。
原发性胃癌肿瘤中VEGF-C的瘤周过表达与LVD增加相关。劳伦肠型癌与VEGF-C过表达相关。肠型胃癌中VEGF-C的过表达与较差的预后相关。
