Ozorio Luísa, Passerini Aline Beatriz Soares, Silva Ana Paula Corradi da, Braga Anna Rafaela Cavalcante, Perrechil Fabiana
Department of Chemical Engineering, Universidade Federal de São Paulo (UNIFESP), Diadema 09913-030, São Paulo, Brazil.
Department of Biosciences, Universidade Federal de São Paulo (UNIFESP), Santos 11015-020, São Paulo, Brazil.
Foods. 2025 May 7;14(9):1645. doi: 10.3390/foods14091645.
Despite the wide variety of plant-based products, developing high-protein products with a desirable texture remains a key challenge for the food industry. Polysaccharide and plant-protein gels offer a cost-effective strategy for meeting the growing demands of vegan and vegetarian markets. This study aimed to develop mixed pea protein-polysaccharide gels with tailored textural properties for plant-based products. The gels were prepared using pea protein and different polysaccharides, including low-acyl gellan gum (GGLA), carrageenan (CA), pectin (PEC), and high-acyl gellan gum (GGHA), along with 60 mM NaCl or CaCl. The dispersions were heated to 80 °C for 30 min under mechanical stirring, followed by a pH adjustment to 7.0 with NaOH (0.1 M). The samples were then analyzed via oscillatory temperature sweep rheometry, confocal microscopy, and uniaxial compression. Self-supporting and non-self-supporting gels were obtained from the various formulations, comprising pure polysaccharide and mixed gels with diverse textures for food applications. The developed gels show a strong potential for use in meat analogs, cheeses, cream cheeses, and sauces, offering the flexibility to fine-tune their mechanical and sensory properties based on the product requirements. Combining biopolymers enables customized texture and functionality, addressing critical gaps in plant-based food innovation.
尽管植物基产品种类繁多,但开发具有理想质地的高蛋白产品仍然是食品行业面临的关键挑战。多糖和植物蛋白凝胶为满足纯素和素食市场不断增长的需求提供了一种经济有效的策略。本研究旨在开发具有定制质地特性的混合豌豆蛋白 - 多糖凝胶,用于植物基产品。凝胶是使用豌豆蛋白和不同的多糖制备的,包括低酰基结冷胶(GGLA)、卡拉胶(CA)、果胶(PEC)和高酰基结冷胶(GGHA),以及60 mM的NaCl或CaCl₂。在机械搅拌下将分散体加热至80°C 30分钟,然后用NaOH(0.1 M)将pH值调节至7.0。然后通过振荡温度扫描流变学、共聚焦显微镜和单轴压缩对样品进行分析。从各种配方中获得了自支撑和非自支撑凝胶,包括纯多糖凝胶和具有不同质地的混合凝胶,可用于食品应用。所开发的凝胶在肉类替代品、奶酪、奶油奶酪和调味汁中具有很强的应用潜力,能够根据产品要求灵活调整其机械和感官特性。将生物聚合物结合使用能够实现定制的质地和功能,填补植物基食品创新中的关键空白。