Sun Cong, Li Weijie, Qi Ruiqiang, Liu Yangming, Wang Xiaoyu, Gong Juwu, Gong Wankui, Pan Jingtao, Li Yang, Shi Yuzhen, Yan Haoliang, Shang Haihong, Yuan Youlu
State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China.
Institute of Millet Research, Shanxi Agricultural University, Changzhi 046011, China.
Int J Mol Sci. 2025 Apr 22;26(9):3944. doi: 10.3390/ijms26093944.
Cotton is a crucial cash crop widely valued for its fiber. It is an important source of natural fiber and has diverse applications. Improving fiber quality is of significant economic and agricultural importance. Purple acid phosphatases (PAPs) are multifunctional enzymes critical for plant cell wall biosynthesis, root architecture modulation, low-phosphorus stress adaptation, and salt/ROS stress tolerance. In this study, a comprehensive genome-wide analysis of the gene family was performed for four cotton species (, , , and ) to explore its potential role in improving fiber quality. A total of 193 genes were identified in these species, revealing several conserved domains that contribute to their functional diversity. Phylogenetic analysis showed that the cotton genes exhibited high homology with , a cell wall synthesis-related gene. Using cotton varieties with contrasting fiber thickness (EZ60, micronaire 4.5 vs. CCRI127, micronaire 3.5), qRT-PCR analysis demonstrated significantly higher expression levels of , , , and in EZ60 fibers during 20-25 DPA compared to CCRI127. These results highlight the potential influence of genes on cotton fiber development and provide valuable insights for improving fiber quality in cotton breeding.
棉花是一种至关重要的经济作物，因其纤维而受到广泛重视。它是天然纤维的重要来源，具有多种用途。提高纤维品质具有重大的经济和农业意义。紫色酸性磷酸酶（PAPs）是多功能酶，对植物细胞壁生物合成、根系结构调控、低磷胁迫适应以及盐/活性氧胁迫耐受性至关重要。在本研究中，对四种棉花物种（、、和）的该基因家族进行了全面的全基因组分析，以探索其在改善纤维品质方面的潜在作用。在这些物种中总共鉴定出193个基因，揭示了几个有助于其功能多样性的保守结构域。系统发育分析表明，棉花基因与一种细胞壁合成相关基因具有高度同源性。使用纤维厚度不同的棉花品种（EZ60，马克隆值4.5与CCRI127，马克隆值3.5），qRT-PCR分析表明，与CCRI127相比，在20 - 25天胚珠发育后期（DPA），EZ60纤维中、、和的表达水平显著更高。这些结果突出了基因对棉花纤维发育的潜在影响，并为棉花育种中改善纤维品质提供了有价值的见解。
