Boamah Solomon, Zhang Shuwu, Xu Bingliang, Zhu Na, Li Enchen
State Key Laboratory of Aridland Crop Science, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China.
Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou 730070, China.
Int J Mol Sci. 2025 Apr 24;26(9):4018. doi: 10.3390/ijms26094018.
(Fp) and soil salinity are two types of stress that interact in complex ways, potentially leading to more severe consequences on wheat growth and productivity. However, little is known about the colonization efficiency and the signal pathways of the beneficial TG1 (TG1) in controlling wheat Fusarium crown rot caused by Fp, and enhancing wheat seedling growth under combined salinity and Fp stresses. Therefore, the present study aims to determine the colonization, phytohormone profile, and signaling pathway in TG1-treated wheat seedlings under salinity and Fp stresses. In a dual culture assay, TG1 exhibited a mycoparasitic effect on Fp growth by coiling, conidial attachment, and parasitism observed under fluorescent microscopy. In addition, TG1 colonized the outermost layers of the wheat seedling roots with biomass consisting of conidia and hyphae. Under 100 mM NaCl stress, the combined TG1+Fp-treated seedlings recorded a control efficacy of 47.01% for the wheat crown rot disease compared with Fp-alone-treated seedlings. The contents of indole-3-acetic acid (IAA), gibberellic acid (GA), abscisic acid (ABA) and jasmonic acid (JA) significantly increased by 72.16%, 86.91%, 20.04%, and 50.40%, respectively, in the combined TG1+Fp treatments, whereas the ethylene (ET) content decreased by 39.07% compared with Fp alone at day 14; and 5.07 and 2.78-fold increases in the expression of salicylic acid (SA) signaling pathway genes, such as pathogenesis-related protein 1 () and isochorismate synthase 1 () genes were recorded respectively, in the combined TG1+Fp-treated seedlings compared with the control at day 14.
尖孢镰刀菌（Fp）和土壤盐分是两种以复杂方式相互作用的胁迫因素，可能对小麦生长和生产力造成更严重的后果。然而，关于有益菌TG1在控制由Fp引起的小麦根腐病以及在盐分和Fp复合胁迫下促进小麦幼苗生长方面的定殖效率和信号通路，人们了解甚少。因此，本研究旨在确定盐分和Fp胁迫下经TG1处理的小麦幼苗中的定殖情况、植物激素谱和信号通路。在共培养试验中，通过荧光显微镜观察到TG1对Fp的生长表现出菌寄生作用，包括缠绕、分生孢子附着和寄生。此外，TG1定殖在小麦幼苗根的最外层，其生物量由分生孢子和菌丝组成。在100 mM NaCl胁迫下，与单独用Fp处理的幼苗相比，TG1 + Fp联合处理的幼苗对小麦根腐病的防治效果为47.01%。在TG1 + Fp联合处理中，吲哚 - 3 - 乙酸（IAA）、赤霉素（GA）、脱落酸（ABA）和茉莉酸（JA）的含量分别显著增加了72.16%、86.91%、20.04%和50.40%，而在第14天，乙烯（ET）含量与单独用Fp处理相比下降了39.07%；与第14天的对照相比，TG1 + Fp联合处理的幼苗中水杨酸（SA）信号通路基因，如病程相关蛋白1（）和异分支酸合成酶1（）基因的表达分别增加了5.07倍和2.78倍。
