Hasan Rakibul, Lv Binna, Uddin Md Jamal, Chen Yingying, Fan Lele, Sun Zhanbin, Sun Manhong, Li Shidong
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Department of Plant Pathology and Seed Science, Sylhet Agricultural University, Sylhet 3100, Bangladesh.
J Fungi (Basel). 2022 May 26;8(6):567. doi: 10.3390/jof8060567.
is an important mycoparasite, with great potential for controlling numerous plant fungal diseases. Understanding the mechanisms and modes of action will assist the development and application of this biocontrol fungus. In this study, the highly efficient 67-1 strain was marked with the green fluorescent protein (GFP), and the transformant possessed the same biological characteristics as the wild-type strain. Fungal interactions with during co-culture and encounter on tomato leaves were assessed by fluorescence confocal and electron microscopy. The results indicated that once the two fungi met, the hyphae of grew alongside those of , then attached tightly to the host and developed special structures, via which the biocontrol fungus penetrated the host and absorbed nutrients, eventually disintegrating the cells of the pathogen. Mycoparasitism to was also observed on tomato leaves, suggesting that can colonize on plants and act following the invasion of the pathogenic fungus.
是一种重要的菌寄生菌，在控制多种植物真菌病害方面具有巨大潜力。了解其作用机制和方式将有助于这种生防真菌的开发和应用。在本研究中，高效的67 - 1菌株用绿色荧光蛋白（GFP）进行标记，转化体具有与野生型菌株相同的生物学特性。通过荧光共聚焦显微镜和电子显微镜评估了两种真菌在番茄叶片上共培养和相遇时的相互作用。结果表明，一旦两种真菌相遇，的菌丝会与的菌丝一起生长，然后紧密附着在寄主上并形成特殊结构，生防真菌通过这些结构穿透寄主并吸收养分，最终使病原菌细胞解体。在番茄叶片上也观察到对的菌寄生现象，这表明可以在植物上定殖，并在致病真菌侵入后发挥作用。
