Zhu Xianxin, Wu Zihan, Deng Xunzhi, Liao Ze, Wang Ruozhong, Luo Zhoufei
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China.
Int J Mol Sci. 2025 May 2;26(9):4337. doi: 10.3390/ijms26094337.
Strigolactones (SLs) are key hormones regulating branching and tillering in rice, impacting plant architecture and yield. A rapid, sensitive, and environmentally friendly method using dispersive liquid-liquid microextraction based on the solidification of floating organic droplets (DLLME-SFO), coupled with ultra-high-performance liquid chromatography and tandem mass spectrometry (UHPLC-MS/MS), has been developed for the determination of three SLs (strigol, orobanchol, and 5-deoxystrigol). The DLLME-SFO method integrates one-step low-temperature extraction and enrichment. The DLLME-SFO conditions were optimized through a single-factor experimental design. Under the best-tested conditions, the developed method exhibited excellent linearity, with the coefficient of determination (R) values greater than 0.9993. The recoveries ranged from 83% to 96%, with precision values ranging from 4.5% to 12.4%. The limits of detection (LODs) varied from 0.6 to 1.2 pg/g fresh weight, indicating the high sensitivity of the method. Additionally, a novel assay protocol for the quantification of SLs in rice in response to nitrogen and phosphorus stress conditions was applied.
独脚金内酯（SLs）是调控水稻分枝和分蘖的关键激素，影响植株形态和产量。基于浮质有机液滴固化的分散液液微萃取（DLLME-SFO），结合超高效液相色谱和串联质谱（UHPLC-MS/MS），开发了一种快速、灵敏且环保的方法，用于测定三种独脚金内酯（独脚金醇、列当醇和5-脱氧独脚金醇）。DLLME-SFO方法集成了一步低温萃取和富集。通过单因素实验设计对DLLME-SFO条件进行了优化。在最佳测试条件下，所开发的方法具有出色的线性，测定系数（R）值大于0.9993。回收率在83%至96%之间，精密度值在4.5%至12.4%之间。检测限（LOD）在0.6至1.2 pg/g鲜重之间，表明该方法具有高灵敏度。此外，还应用了一种新的检测方案来定量水稻在氮磷胁迫条件下的独脚金内酯。
