Jappe Uta, Kolaly Tahmina, de Vries Mareike S, Gülsen Askin, Homann Arne
Division of Clinical and Molecular Allergology, Priority Research Area Chronic Lung Diseases, Research Center Borstel, Leibniz Lung Center, Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), 23845 Borstel, Germany.
Interdisciplinary Allergy Outpatient Clinic, Department of Pneumology, UKSH Campus Lübeck, University of Lübeck, 23538 Lübeck, Germany.
Nutrients. 2025 Apr 30;17(9):1541. doi: 10.3390/nu17091541.
BACKGROUND/OBJECTIVES: Sensitization to the carbohydrate antigen α-Gal is associated with allergic reactions against different types of food that contain α-Gal (e.g., mammalian meat). This form of allergy is termed α-Gal syndrome (AGS), and the diagnosis is challenging due to delayed symptom onset and cross-reactivity with multiple mammalian products. It is estimated that AGS is underdiagnosed, pointing to an unmet need for patient care.
Sera from patients with suspected AGS based on clinical history were analyzed by ImmunoCAP and the IgE cross-reactivity immune profiling (ICRIP) system specifically developed by us. IgE from patient sera against different forms of α-Gal was analyzed using α-Gal-containing analytes and negative controls.
Sera from 33 patients with suspected AGS were analyzed. Sera from 22 patients yielded a clearly positive signal (>0.35 kU/L) for IgE against α-Gal in ImmunoCAP. For 7 of the remaining 11 patients with negative or ambiguous (IgE level between 0.1 and 0.35 kU/L) results in ImmunoCAP, ICRIP analyses supported the suspected association of the allergy symptoms with IgE against α-Gal components. This component-resolved analysis helps the allergist to provide an individual diagnosis for each patient.
The diagnosis of AGS is challenging. An interplay between clinical history and lab analysis via ImmunoCAP and the specifically developed ICRIP system helps patients and allergists in establishing the correct diagnosis, thereby preventing accidental exposure and recurrent AGS episodes.
背景/目的：对碳水化合物抗原α - 半乳糖的致敏与针对不同类型含α - 半乳糖食物（如哺乳动物肉类）的过敏反应相关。这种过敏形式被称为α - 半乳糖综合征（AGS），由于症状出现延迟以及与多种哺乳动物产品存在交叉反应，其诊断具有挑战性。据估计，AGS存在诊断不足的情况，这表明患者护理方面存在未满足的需求。
通过ImmunoCAP以及我们专门开发的IgE交叉反应免疫谱分析（ICRIP）系统，对基于临床病史疑似患有AGS的患者血清进行分析。使用含α - 半乳糖的分析物和阴性对照，分析患者血清中针对不同形式α - 半乳糖的IgE。
对33例疑似AGS患者的血清进行了分析。在ImmunoCAP检测中，22例患者的血清针对α - 半乳糖的IgE产生了明显阳性信号（>0.35 kU/L）。在ImmunoCAP检测中，其余11例结果为阴性或不明确（IgE水平在0.1至0.35 kU/L之间）的患者中，有7例通过ICRIP分析支持了过敏症状与针对α - 半乳糖成分的IgE之间的疑似关联。这种成分解析分析有助于过敏症专科医生为每位患者提供个体化诊断。
AGS的诊断具有挑战性。临床病史与通过ImmunoCAP和专门开发的ICRIP系统进行的实验室分析之间的相互作用，有助于患者和过敏症专科医生做出正确诊断，从而防止意外接触和AGS复发。
