Wang Min, Zhao Lin, Wang Yumei, Zhang Chengfeng, Li He
Key Laboratory of Geriatric Nutrition and Health, Beijing Technology and Business University, Beijing 100048, China.
The Product Makers Co., Ltd., Shanghai 200444, China.
Nutrients. 2025 May 5;17(9):1589. doi: 10.3390/nu17091589.
BACKGROUND/OBJECTIVE: The prevention and treatment of alcoholic liver disease (ALD) urgently require safe and effective nutritional intervention strategies. Polyphenol extracts from sugarcane molasses (SP) show antioxidant and anti-inflammatory potential, yet their protective effects against ALD have not been elucidated. This study explored the therapeutic potential of SP in alcohol-induced chronic liver damage.
A graded alcohol concentration-induced liver damage model was established in C57BL/6J mice to systematically evaluate SP's regulatory effects on liver function markers, lipid metabolism, oxidative stress indicators, inflammatory factors, and related molecular mechanisms through a 10-week nutritional intervention.
The results demonstrated that SP intervention significantly inhibited the liver index, alanine aminotransferase and aspartate aminotransferase activities, and triglyceride and total cholesterol accumulation in mice. SP enhanced antioxidant enzyme activities in a dose-dependent manner, with the high-dose group increasing catalase activity by 161.19% and superoxide dismutase activity by 22.97%. Furthermore, SP significantly reduced the levels of pro-inflammatory cytokines, including interleukin-1β, interleukin-6, and tumor necrosis factor-α, thereby alleviating hepatic inflammatory infiltration. Mechanistic studies revealed that SP effectively mitigated alcohol-induced oxidative stress and inflammatory injury by inhibiting cytochrome P450 2E1 overexpression, regulating the Kelch-like ECH-associated protein 1 signaling pathway, and suppressing nuclear factor-kappa B pathway activation.
The findings reveal that SP mitigates ALD via synergistic antioxidant and anti-inflammatory mechanisms, providing a novel strategy for high-value utilization of sugarcane molasses byproducts in agricultural industries. Future studies should focus on the contribution of the different phenolics in SP and validate their specific hepatoprotective mechanisms.
背景/目的：酒精性肝病（ALD）的防治迫切需要安全有效的营养干预策略。甘蔗废蜜多酚提取物（SP）具有抗氧化和抗炎潜力，但其对ALD的保护作用尚未阐明。本研究探讨了SP对酒精性慢性肝损伤的治疗潜力。
在C57BL/6J小鼠中建立梯度酒精浓度诱导的肝损伤模型，通过为期10周的营养干预，系统评价SP对肝功能指标、脂质代谢、氧化应激指标、炎症因子及相关分子机制的调节作用。
结果表明，SP干预显著抑制了小鼠的肝脏指数、丙氨酸氨基转移酶和天冬氨酸氨基转移酶活性，以及甘油三酯和总胆固醇的蓄积。SP以剂量依赖的方式增强抗氧化酶活性，高剂量组过氧化氢酶活性增加161.19%，超氧化物歧化酶活性增加22.97%。此外，SP显著降低了促炎细胞因子的水平，包括白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α，从而减轻了肝脏炎症浸润。机制研究表明，SP通过抑制细胞色素P450 2E1过表达、调节 Kelch样ECH相关蛋白1信号通路和抑制核因子-κB通路激活，有效减轻酒精诱导的氧化应激和炎症损伤。
研究结果表明，SP通过协同抗氧化和抗炎机制减轻ALD，为农业产业中甘蔗废蜜副产品的高值化利用提供了新策略。未来的研究应聚焦于SP中不同酚类物质的贡献，并验证其具体的肝脏保护机制。
