Vargas Daniel, Peña Daniel, Whitehead Emma, Grayson Warren L, Le Monnier Benjamin P, Tsapatsis Michael, Romero-Hasler Patricio, Orellana Rocío, Neira Miguel, Covarrubias Cristian
Laboratory of Nanobiomaterials, Institute for Research in Dental Sciences, Faculty of Dentistry, University of Chile, Santiago 8320000, Chile.
Department of Biomedical Engineering, School of Medicine, Institute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD 21201, USA.
Materials (Basel). 2025 May 2;18(9):2091. doi: 10.3390/ma18092091.
The development of biomaterials that enhance bone healing and integrate with native bone tissue has gained significant interest. Metal-organic frameworks (MOFs) have emerged as promising candidates due to their unique surface properties and biocompatibility. While various bioactive element-incorporated MOFs have been studied, the osteogenic potential of lithium (Li)-modified MOFs remains largely unexplored. This study presents the synthesis and characterization of a nanosized calcium-based MOF incorporating Li⁺ ions to enhance osteoinductive properties. The MOFs were evaluated in vitro for apatite mineralization, degradation, ion release, protein adsorption, cell adhesion, viability, and osteogenic differentiation using pre-osteoblast cells. The synthesized MOFs promoted apatite formation under simulated physiological conditions, facilitated by their surface nucleation properties, controlled degradation, and sustained Li and Ca ion release. Cytocompatibility assays confirmed excellent pre-osteoblast adhesion and viability. Furthermore, CaMOF nanoparticles stimulated osteogenic differentiation by enhancing alkaline phosphatase (ALP) activity, even in the absence of osteogenic supplements. Among tested MOFs, Li/CaMOF exhibited the highest osteoinductive potential. These findings highlight lithium-modified MOFs as promising biomaterials for bone regeneration. However, further in vivo studies are necessary to assess their long-term stability, bone integration, and clinical applicability.
开发能够促进骨愈合并与天然骨组织整合的生物材料已引起广泛关注。金属有机框架（MOF）因其独特的表面性质和生物相容性而成为有前景的候选材料。虽然已对各种掺入生物活性元素的MOF进行了研究，但锂（Li）改性MOF的成骨潜力在很大程度上仍未得到探索。本研究介绍了一种掺入Li⁺离子以增强骨诱导性能的纳米级钙基金属有机框架的合成与表征。使用前成骨细胞对该金属有机框架进行了体外磷灰石矿化、降解、离子释放、蛋白质吸附、细胞黏附、活力和成骨分化评估。合成的金属有机框架在模拟生理条件下促进了磷灰石形成，这得益于其表面成核特性、可控降解以及Li和Ca离子的持续释放。细胞相容性检测证实了前成骨细胞具有良好的黏附性和活力。此外，即使在没有成骨补充剂的情况下，钙基金属有机框架纳米颗粒也通过增强碱性磷酸酶（ALP）活性刺激了成骨分化。在测试的金属有机框架中，锂/钙基金属有机框架表现出最高的骨诱导潜力。这些发现突出了锂改性金属有机框架作为骨再生有前景的生物材料的地位。然而，需要进一步的体内研究来评估它们的长期稳定性、骨整合和临床适用性。
