基于β-内酰胺酶/转肽酶样超家族蛋白质结构催化核心比较的α和ω分类

Denesyuk Alexander I, Denessiouk Konstantin, Johnson Mark S, Uversky Vladimir N

Structural Bioinformatics Laboratory, Biochemistry, InFLAMES Research Flagship Center, Faculty of Science and Engineering, Åbo Akademi University, 20520 Turku, Finland.

Department of Molecular Medicine and USF Health Byrd Alzheimer's Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA.

Molecules. 2025 Apr 30;30(9):2019. doi: 10.3390/molecules30092019.

β-Lactamase/transpeptidase-like superfamily proteins are serine proteases that use the Ser-Lys catalytic dyad to carry out their biological functions. Here, we investigate the three known families of β-lactamase/transpeptidase-like superfamily proteins, β-lactamase/D-Ala carboxypeptidase, glutaminase, and Dac-like, and describe the structural catalytic cores that govern the catalytic residues in these proteins. We show that the structural catalytic core of these proteins is a combination of three zones, the mutual three-dimensional arrangement of which correspondingly determines their belonging to one of seven and twenty-four established groups and subgroups.

β-内酰胺酶/转肽酶样超家族蛋白是丝氨酸蛋白酶，利用丝氨酸-赖氨酸催化二元体来执行其生物学功能。在此，我们研究了β-内酰胺酶/转肽酶样超家族蛋白的三个已知家族，即β-内酰胺酶/D-丙氨酸羧肽酶、谷氨酰胺酶和类Dac蛋白，并描述了控制这些蛋白中催化残基的结构催化核心。我们表明，这些蛋白的结构催化核心是三个区域的组合，它们的相互三维排列相应地决定了它们属于七个既定组和二十四个亚组中的哪一组。