Shi Jianghua, Yu Huasheng, Liu Renhu, Zhang Yaofeng, Fu Ying, Wang Tanliu, Ni Xiyuan, Zheng Tao, Zhao Jianyi
Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Science, Hangzhou 310000, China.
Institute of Biotechnology, Zhejiang Academy of Agricultural Science, Hangzhou 310000, China.
Plants (Basel). 2025 May 6;14(9):1397. doi: 10.3390/plants14091397.
A male sterile mutant, S201, was identified in . Genetic analysis revealed that the male sterility trait was controlled by a recessive nuclear gene, (), which was stably inherited. The results of microscopy showed that the main reason for male sterility was a defect in microspore development, resulting in the absence of typical exine and mature microspores. Bulked segregant analysis (BSA) and genotyping of an F population showed that the gene was located in a 1.4 Mb region. Sequence analysis showed that the gene in this region contained two non-synonymous SNPs, leading to substitutions of two amino acids. A high-throughput KASP marker was characterized to detect the presence of the gene in the breeding population. The data presented here indicate that the male sterile mutant S201 can be applied in rapeseed breeding by producing the male sterile line and that the KASP marker developed for male sterility will be useful in marker-assisted selection of male sterile individuals in rapeseed-breeding programs.
在……中鉴定出一个雄性不育突变体S201。遗传分析表明，雄性不育性状由一个隐性核基因（）控制，该基因可稳定遗传。显微镜观察结果显示，雄性不育的主要原因是小孢子发育缺陷，导致缺乏典型的外壁和成熟小孢子。混合分离群体分析法（BSA）和一个F群体的基因分型表明，该基因位于一个1.4 Mb的区域。序列分析表明，该区域的基因包含两个非同义单核苷酸多态性（SNP），导致两个氨基酸发生替换。鉴定出一个高通量竞争性等位基因特异性PCR（KASP）标记，用于检测育种群体中该基因的存在。本文提供的数据表明，雄性不育突变体S201可通过培育雄性不育系应用于油菜育种，并且为雄性不育开发的KASP标记将有助于在油菜育种计划中对雄性不育个体进行标记辅助选择。
