通过磷酸纤维素柱色谱法分离和纯化高迁移率族蛋白（HMG蛋白）以及组蛋白H1、H5和核心组蛋白。
Isolation and separation of HMG proteins and histones H1 and H5 and core histones by column chromatography on phosphocellulose.
作者信息
Garg L C, Reeck G R
机构信息
Gene Regulation Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India.
出版信息
Protein Expr Purif. 1998 Nov;14(2):155-9. doi: 10.1006/prep.1998.0927.
A single-step method to dissociate histones as well as nonhistone chromosomal proteins from chicken erythrocyte chromatin and their separation into histones H1, H5, core histones, and high mobility group proteins by column chromatography on phosphocellulose is presented. NaCl at 2.0 M is effective in dissociating both histones and nonhistone proteins. The core histones elute as a complex. The pH is a critical factor in separating H5 from the core histones.
本文介绍了一种从鸡红细胞染色质中分离组蛋白以及非组蛋白染色体蛋白的单步方法，以及通过磷酸纤维素柱色谱将它们分离为组蛋白H1、H5、核心组蛋白和高迁移率族蛋白的方法。2.0 M的NaCl可有效分离组蛋白和非组蛋白。核心组蛋白以复合物形式洗脱。pH是将H5与核心组蛋白分离的关键因素。
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