Liu Jinping, Bu Xiaoyu, Yang Zikang, Yang Linshuai, Ai Qi, Gao Baoxiang
Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, P. R. China.
College of Optical and Electronic Technology, China Jiliang University, Hangzhou, 310018, P. R. China.
Chemistry. 2025 Jun 23;31(35):e202500772. doi: 10.1002/chem.202500772. Epub 2025 May 24.
In the complex cellular microenvironment, lipid droplets (LDs) and the endoplasmic reticulum (ER) are essential for maintaining cellular homeostasis. Their interactions are crucial for regulating processes such as lipid metabolism. Nevertheless, simultaneously monitoring these two organelles remains a challenging task. In this study, a dual-organelle-targeted perylene diimides (PDIs) based probe, LD-ER-PDI, by incorporating a 2-azetidinone substituent in the bay-region and two distinct targeting groups for LDs and ER at opposite ends of the imide positions was developed, enabling discrimination between LDs and ER. Moreover, it can effectively visualize the dynamic behaviors of LDs and ER under starvation and oleic acid-treated conditions, and also allows real-time monitoring of LDs formation from the ER. This work not only provides a valuable tool for studying the dynamic interactions between LDs and ER but also highlights a strategy for designing dual-organelle-targeted fluorescent probes.
在复杂的细胞微环境中，脂滴（LDs）和内质网（ER）对于维持细胞内稳态至关重要。它们之间的相互作用对于调节脂质代谢等过程至关重要。然而，同时监测这两个细胞器仍然是一项具有挑战性的任务。在本研究中，通过在湾区引入2-氮杂环丁酮取代基，并在酰亚胺位置的两端引入两个针对脂滴和内质网的不同靶向基团，开发了一种基于双细胞器靶向苝二亚胺（PDIs）的探针LD-ER-PDI，能够区分脂滴和内质网。此外，它可以有效地可视化饥饿和油酸处理条件下脂滴和内质网的动态行为，还可以实时监测内质网中脂滴的形成。这项工作不仅为研究脂滴和内质网之间的动态相互作用提供了一个有价值的工具，还突出了一种设计双细胞器靶向荧光探针的策略。
