虾蟹过敏相关差异表达蛋白的分析
Analysis of Differentially Expressed Proteins Involved in Shrimp and Crab Allergies.
作者信息
Tan Ruhong, Wei Xiwen, Zhong Jiacheng, Wang Yuanbin, Yu Qing
机构信息
Department of Clinical Laboratory, Foshan Women and Children Hospital, Foshan, Guangdong, P. R. China.
Department of Clinical Laboratory, Shenzhen Mental Health Center/Shenzhen Kangning Hospital, Shenzhen, Guangdong, P. R. China.
出版信息
J Clin Lab Anal. 2025 Jun;39(12):e70053. doi: 10.1002/jcla.70053. Epub 2025 May 14.
BACKGROUND
Shrimp and crab allergies have garnered increasing attention in recent years. Unlike many other food allergies, they are less likely to be outgrown by children and tend to trigger more severe allergic symptoms. The underlying mechanisms that lead to these phenomena have not yet been fully elucidated.
METHODS
We used proteomics iTRAQ technology to identify differentially expressed proteins in shrimp and crab allergic patients and normal controls.
RESULTS
Ninety differentially expressed proteins, including 82 upregulated proteins and 8 downregulated proteins, were identified. Furthermore, MRC2 was validated to be upregulated in shrimp and crab allergic patients by ELISA.
CONCLUSION
These findings have established a comprehensive proteomics map of shrimp and crab allergies, laying the foundation for further analysis of the pathogenesis and regulatory network of shrimp and crab allergies.
背景
近年来，虾类和蟹类过敏日益受到关注。与许多其他食物过敏不同，儿童对虾蟹过敏不太可能随着年龄增长而自愈，且往往会引发更严重的过敏症状。导致这些现象的潜在机制尚未完全阐明。
方法
我们使用蛋白质组学iTRAQ技术，鉴定虾类和蟹类过敏患者及正常对照中差异表达的蛋白质。
结果
共鉴定出90种差异表达蛋白质，其中包括82种上调蛋白和8种下调蛋白。此外，通过酶联免疫吸附测定法（ELISA）验证了虾类和蟹类过敏患者中巨噬细胞甘露糖受体C2（MRC2）上调。
结论
这些发现建立了虾类和蟹类过敏的综合蛋白质组学图谱，为进一步分析虾类和蟹类过敏的发病机制及调控网络奠定了基础。
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