Habib Syed Sikandar, Majeed Saima, Rind Khalid Hussain, Naz Saira, Acar Ümit, Cravana Cristina, Ullah Mujeeb, Khan Khalid, Zahid Muhammad, Mohany Mohamed, Fazio Francesco
Department of Zoology, University of Sargodha, Sargodha, Punjab, Pakistan.
Department of Maritime Sciences, Bahria University Karachi Campus, Karachi, Sindh, Pakistan.
J Aquat Anim Health. 2025 Jun 13;37(2):85-96. doi: 10.1093/jahafs/vsaf006.
In aquaculture, the trend is shifting towards using plant-derived alternatives that are abundant in phytochemicals as effective replacements for traditional antibiotics and synthetic feed additives. In the present study, the effects of tulsi Ocimum sanctum extract on growth performance, hemato-biochemical indices, serum immune parameters, and antioxidant parameters in Common Carp Cyprinus carpio were investigated.
Common Carp (mean body weight ± SD = 10.6 ± 0.13 g) were fed experimental diets that contained tulsi leaf extract at 0.0 (control), 0.5, 1.0, and 1.5% for 60 d (25 fish/treatment).
The findings revealed a considerable enhancement in growth performance and a decreased feed conversion ratio, especially for the 1.0% tulsi-based diet. Additionally, weight gain and feed conversion ratio exhibited significance at both the linear and quadratic levels, as indicated by polynomial contrasts. The hematological and biochemical profiles exhibited improvements in groups receiving tulsi-enriched diets. The antioxidant status of fish serum exhibited a notable increase, as evidenced by elevated activities of total antioxidant capacity, superoxide dismutase, and catalase in fish that received the 1.0% and 1.5% tulsi-based diets. Tulsi-supplemented diets led to remarkable enhancements in serum lysozyme activity, alternative complement activity, and total immunoglobulin content. Moreover, tulsi supplementation at 1.0% and 1.5% showcased a significant reduction in serum glucose and cortisol levels compared to the other groups.
In conclusion, tulsi extract emerged as a valuable asset, positively influencing growth, blood parameters, antioxidant balance, and serum immune response in Common Carp, particularly at supplementation levels ranging from 1.0% to 1.5% in the diet.
在水产养殖中，趋势正转向使用富含植物化学物质的植物源替代品，以有效替代传统抗生素和合成饲料添加剂。在本研究中，调查了圣罗勒提取物对鲤鱼生长性能、血液生化指标、血清免疫参数和抗氧化参数的影响。
将平均体重±标准差 = 10.6 ± 0.13 g的鲤鱼投喂含0.0%（对照）、0.5%、1.0%和1.5%圣罗勒叶提取物的实验饲料，持续60天（每组25尾鱼）。
研究结果显示生长性能有显著提高，饲料转化率降低，尤其是1.0%圣罗勒基饲料组。此外，如多项式对比所示，增重和饲料转化率在线性和二次水平上均表现出显著性。接受富含圣罗勒饲料的组血液学和生化指标有所改善。鱼血清的抗氧化状态显著增加，接受1.0%和1.5%圣罗勒基饲料的鱼中总抗氧化能力、超氧化物歧化酶和过氧化氢酶活性升高证明了这一点。添加圣罗勒的饲料使血清溶菌酶活性、替代补体活性和总免疫球蛋白含量显著提高。此外，与其他组相比，添加1.0%和1.5%圣罗勒的饲料组血清葡萄糖和皮质醇水平显著降低。
总之，圣罗勒提取物是一种有价值的物质，对鲤鱼的生长、血液参数、抗氧化平衡和血清免疫反应有积极影响，尤其是在饲料中添加水平为1.0%至1.5%时。
