利用病毒诱导基因沉默技术解析与烟草花冠脱落相关的本氏烟草基因的功能

Virus-Induced Gene Silencing to Unravel the Function of Nicotiana benthamiana Genes Linked to Corolla Abscission.

作者信息

Velázquez Karelia, Vives Mari Carmen, Ruiz-Ruiz Susana, Guerri José, Ventimilla Daniel, Terol Javier, Pérez-Amador Miguel A, Talón Manuel, Tadeo Francisco R

机构信息

Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Moncada (Valencia), Spain.

Centro de Genómica, Instituto Valenciano de Investigaciones Agrarias, Moncada (Valencia), Spain.

出版信息

Methods Mol Biol. 2025;2916:139-151. doi: 10.1007/978-1-0716-4470-6_13.

DOI:10.1007/978-1-0716-4470-6_13

PMID:40366593

Abstract

Virus-induced gene silencing (VIGS) is a powerful and cost-effective biotechnological tool for studying gene function in plants. It allows for rapid downregulation of gene expression without the need for tissue culture or genetic transformation. While VIGS is transient and may pose challenges for certain developmental genes, it has been successfully used to study organ abscission. Using a VIGS protocol based on Citrus leaf blotch virus (CLBV) viral vectors, we were able to characterize the regulatory role of INFLORESCENCE DEFICIENT IN ABSCISSION (IDA)-like genes and their potential HAESA (HAE)-like receptors in the process of corolla abscission in Nicotiana benthamiana flowers.

摘要

病毒诱导的基因沉默（VIGS）是一种用于研究植物基因功能的强大且经济高效的生物技术工具。它无需组织培养或遗传转化就能快速下调基因表达。虽然VIGS是短暂的，可能对某些发育基因构成挑战，但它已成功用于研究器官脱落。使用基于柑橘叶斑驳病毒（CLBV）病毒载体的VIGS方案，我们能够表征拟南芥花中脱落缺陷花序（IDA）样基因及其潜在的HAESA（HAE）样受体在花冠脱落过程中的调控作用。