Wei Xiangcong, Huang Xinlong, Huang Yafei, Su Ting, Duan Qiuting, Wan Jinxiu, Sun Yi, Xu Ya
Medical School, Kunming University of Science and Technology, Kunming, PR China; Department of Clinical Laboratory, the First People's Hospital of Yunnan Province, Kunming, PR China; The Affiliated Hospital of Kunming University of Science and Technology, The First People's Hospital of Yunnan Province, Kunming, PR China.
Department of Clinical Laboratory, the First People's Hospital of Yunnan Province, Kunming, PR China; The Affiliated Hospital of Kunming University of Science and Technology, The First People's Hospital of Yunnan Province, Kunming, PR China.
Diagn Microbiol Infect Dis. 2025 Sep;113(1):116896. doi: 10.1016/j.diagmicrobio.2025.116896. Epub 2025 May 10.
Sexually transmitted infections (STIs) such as human papillomavirus (HPV), Ureaplasma urealyticum (UU), Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and herpes simplex virus type 2 (HSV-2) significantly affect male reproductive health. Coinfections among these pathogens may aggravate disease severity, yet data on their prevalence among male outpatients remain scarce. In this study, we investigated the prevalence of UU, HSV2, NG, CT, and HPV infections and coinfections in the external genitalia of male outpatients in Kunming, Yunnan. HPV genotyping was performed using PCR and Sanger sequencing, while other pathogens were detected using real-time quantitative PCR. Relevant medical records were obtained from the hospital information system. Statistical analyses were conducted to examine associations between coinfections and clinical characteristics. The prevalence rates for UU, HSV-2, NG, CT, and HPV infections in the external genitalia of male outpatients were 33.31 %, 27.04 %, 23.11 %, 11.70 %, and 8.25 %, respectively. The most common coinfection was NG+UU (14.81 %), followed by CT+UU (4.02 %) and CT+NG (1.45 %). Coinfection rates for HSV2 with UU, CT, and NG were 5.35 %, 0.58 %, and 2.03 %, respectively. HPV coinfection rates with UU, CT, NG, and HSV2 were 3.30 %, 0.62 %, 3.57 %, and 0.00 %, respectively. The five most common HPV subtypes were HPV 43, 52, 56, 58, and 33. This study highlights the high prevalence of STIs and coinfections among male outpatients in Kunming, underscoring the urgent need for improved screening and prevention strategies to address these widespread health concerns.
性传播感染（STIs），如人乳头瘤病毒（HPV）、解脲脲原体（UU）、沙眼衣原体（CT）、淋病奈瑟菌（NG）和2型单纯疱疹病毒（HSV-2），会显著影响男性生殖健康。这些病原体之间的合并感染可能会加重疾病的严重程度，但关于它们在男性门诊患者中的流行情况的数据仍然很少。在本研究中，我们调查了云南昆明男性门诊患者外生殖器中UU、HSV2、NG、CT和HPV感染及合并感染的流行情况。使用聚合酶链反应（PCR）和桑格测序进行HPV基因分型，而其他病原体则使用实时定量PCR进行检测。从医院信息系统获取相关医疗记录。进行统计分析以检查合并感染与临床特征之间的关联。男性门诊患者外生殖器中UU、HSV-2、NG、CT和HPV感染的患病率分别为33.31%、27.04%、23.11%、11.70%和8.25%。最常见的合并感染是NG+UU（14.81%），其次是CT+UU（4.02%）和CT+NG（1.45%）。HSV2与UU、CT和NG的合并感染率分别为5.35%、0.58%和2.03%。HPV与UU、CT、NG和HSV2的合并感染率分别为3.30%、0.62%、3.57%和0.00%。五种最常见的HPV亚型是HPV 43、52、56、58和33。本研究强调了昆明男性门诊患者中性传播感染和合并感染的高患病率，突出了迫切需要改进筛查和预防策略以解决这些广泛的健康问题。
