Rashid Rozina, Ejaz Uroosa, Alhilfi Wissal Audah, Alorabi Mohammed, Ali Syed Tariq, Sohail Muhammad
Department of Microbiology, University of Karachi, Karachi, 75270, Pakistan.
Department of Microbiology, University of Balochistan, Quetta, Pakistan.
BMC Biotechnol. 2025 May 14;25(1):37. doi: 10.1186/s12896-025-00974-6.
Biomass from halophytes is considered as a promising chemical feedstock. Its bioconversion to obtain reducing sugars and to concomitantly improve antioxidant potential has been described less frequently. This is the first report describing application of xylanase from Neobacillus sedimentimangrovi for the saccharification of Ipomoea pes-caprae (IPC) and Suaeda fruticosa (SF). In this study, the biomass IPC and SF was separately or co-pretreated by freeze-thaw and 1% HSO. Results showed that significant amount of reducing sugar was obtained by saccharification of acid and freeze-thaw pretreated IPC (44 mg g) and freeze-thaw pretreated SF (43 mg g). The residues after saccharification were also analyzed for their antioxidant potential where IPC residues exhibited 1.13 folds higher potential than that of SF. Antioxidant potential (83.9%) was obtained when purified xylanase was used for the saccharification of IPC. Moreover, absence of lignin-related peaks in the NMR and IR spectra of the treated substrates indicated efficient delignification. The characteristic peaks of the hemicellulosic fractions in saccharified samples were also disturbed, indicating changes in the crystallinity of the substrates. The SEM images and spectra of the saccharified substrates clearly indicated the degradation of hemicellulosic content by xylanse.
盐生植物的生物质被认为是一种有前途的化学原料。关于将其生物转化以获得还原糖并同时提高抗氧化潜力的描述较少。这是第一篇描述来自红树林新芽孢杆菌的木聚糖酶用于海刀豆（IPC）和碱蓬（SF）糖化的报告。在本研究中，生物质IPC和SF分别或共同通过冻融和1%硫酸进行预处理。结果表明，通过对酸和冻融预处理的IPC（44毫克/克）和冻融预处理的SF（43毫克/克）进行糖化，获得了大量还原糖。糖化后的残渣也进行了抗氧化潜力分析，其中IPC残渣的潜力比SF高1.13倍。当使用纯化的木聚糖酶对IPC进行糖化时，获得了83.9%的抗氧化潜力。此外，处理后底物的核磁共振和红外光谱中没有木质素相关峰，表明脱木质素效果良好。糖化样品中半纤维素部分的特征峰也受到干扰，表明底物结晶度发生了变化。糖化底物的扫描电子显微镜图像和光谱清楚地表明木聚糖酶对半纤维素含量的降解作用。
