Shen Liling, Yao Jia
Department of Dermatology, Zhejiang Provincial Dermatology Hospital, Huzhou, China.
J Cosmet Dermatol. 2025 May;24(5):e70087. doi: 10.1111/jocd.70087.
Margarita (pearl) has detoxifying and skin barrier-repairing properties. The study was to evaluate the therapeutic effect of pearl on melasma and to preliminarily explore its possible mechanism of action.
The efficacy of pearl on patients with melasma was evaluated by the melasma area and severity index (MASI) and physician's global assessment (PGA) score. DNA sequencing was performed on pharyngeal swab samples from patients with melasma to obtain gene loci related to melasma. The active compounds and potential targets for pearl were retrieved from Integrated Traditional Chinese Medicine and HERB databases. The relevant genes of melasma were obtained from the GeneCards database and intersected with the targets of pearl to identify potential targets of pearl against melasma. The potential targets were mapped for the KEGG pathway in the KEGG Mapper and verified using human melanoma A375 cells that were exposed to ultraviolet irradiation.
Pearl significantly improved the MASI and PGA scores of patients with melasma. DNA sequencing revealed that TYR and DCT were genes related to melasma. Calcium carbonate, iron, magnesium, manganese, silicon, strontium, and zinc were active compounds for pearl. CAMP-responsive element binding protein 1 (CREB1) was the target of pearl against melasma. Pearl regulated microphthalmia-associated transcription factors through CREB and affected melasma-related genes TYR and DCT, which in turn inhibit melanoma cell activity and intracellular tyrosinase activity.
Pearl can alleviate melasma by targeting the CREB1/MITF axis and then the melasma-related gene loci TYR and DCT.
玛格丽塔（珍珠）具有解毒和修复皮肤屏障的特性。本研究旨在评估珍珠对黄褐斑的治疗效果，并初步探讨其可能的作用机制。
采用黄褐斑面积和严重程度指数（MASI）及医生整体评估（PGA）评分来评估珍珠对黄褐斑患者的疗效。对黄褐斑患者的咽拭子样本进行DNA测序，以获得与黄褐斑相关的基因位点。从中药综合数据库和HERB数据库中检索珍珠的活性成分和潜在靶点。从GeneCards数据库中获取黄褐斑的相关基因，并与珍珠的靶点进行交叉分析，以确定珍珠抗黄褐斑的潜在靶点。在KEGG Mapper中对潜在靶点进行KEGG通路映射，并使用暴露于紫外线照射的人黑色素瘤A375细胞进行验证。
珍珠显著改善了黄褐斑患者的MASI和PGA评分。DNA测序显示，TYR和DCT是与黄褐斑相关的基因。碳酸钙、铁、镁、锰、硅、锶和锌是珍珠的活性成分。环磷酸腺苷反应元件结合蛋白1（CREB1）是珍珠抗黄褐斑的靶点。珍珠通过CREB调节小眼相关转录因子，并影响黄褐斑相关基因TYR和DCT，进而抑制黑色素瘤细胞活性和细胞内酪氨酸酶活性。
珍珠可通过靶向CREB1/MITF轴，进而作用于黄褐斑相关基因位点TYR和DCT来减轻黄褐斑。
