Kutteh W H, Welander C E, Homesley H D, Doellgast G J
Am J Obstet Gynecol. 1985 Sep 15;153(2):124-9. doi: 10.1016/0002-9378(85)90094-8.
Cyst and ascites fluids from patients with ovarian epithelial neoplasms contain large amounts of soluble immunoglobulins without detectable antibody activity against human ovarian tumor cell lines by indirect immunofluorescence. Membrane fragments were prepared from 56 human ovarian effusions, and the presence of membrane-bound IgG, IgA, and IgM was demonstrated. The predominant membrane-bound immunoglobulin class was IgG, which ranged from 18 to 4275 ng/ml on samples tested, whereas IgA was present in the range of 5 to 52 ng/ml. The autologous membrane-bound antibodies strongly recognized cell-surface antigens on four human ovarian cell lines and four surgical specimens of human ovarian adenocarcinoma but did not react with normal human ovaries, non-ovarian normal and neoplastic tissues, or non-ovarian human cell lines by indirect immunofluorescence assay. These studies indicate that patients with ovarian cancer have the capability of recognizing and forming antibodies against autologous ovarian tumor-associated antigens.
卵巢上皮性肿瘤患者的囊肿液和腹水含有大量可溶性免疫球蛋白，但通过间接免疫荧光法未检测到针对人卵巢肿瘤细胞系的抗体活性。从56份人卵巢积液中制备膜碎片，证实存在膜结合的IgG、IgA和IgM。主要的膜结合免疫球蛋白类别是IgG，在所检测的样本中其含量范围为18至4275 ng/ml，而IgA的含量范围为5至52 ng/ml。自体膜结合抗体通过间接免疫荧光测定法强烈识别四种人卵巢细胞系和四个人卵巢腺癌手术标本上的细胞表面抗原，但不与正常人卵巢、非卵巢正常和肿瘤组织或非卵巢人细胞系发生反应。这些研究表明，卵巢癌患者有能力识别并形成针对自体卵巢肿瘤相关抗原的抗体。
