Liu Yuming, Bai Hua, Qiu Huitong, Fei Dongliang, Ma Mingxiao
College of Animal Husbandry and Veterinary, Jinzhou Medical University, Jinzhou, China.
Front Microbiol. 2025 Apr 30;16:1563240. doi: 10.3389/fmicb.2025.1563240. eCollection 2025.
Chinese sacbrood virus (CSBV) is highly lethal to honeybee larvae (especially the larva of ) and causes considerable losses to beekeeping industry. N6-methyladenine (m6A) modification of mRNA is a predominant post-transcriptional modification in eukaryotes and plays a role in viral infection. However, the role of m6A modification in CSBV infection remains unclear. Herein, we performed high-throughput sequencing for m6A-seq in CSBV-infected and non-infected larvae to investigate host transcriptome-wide m6A modifications and identify m6A-modified genes. A total of 671 variant peaks were identified. Combined analysis of m6A modification and mRNA expression revealed that a significant correlation between mRNA methylation modifications and expression levels observed for 668 Genes. It was proved that CSBV infection can cause important m6A modification changes in host. We examined the effects of CSBV infection on expression of two methylation regulatory genes by qPCR. At the same time, we verified the effect of two methylation regulatory genes on CSBV replication using RNAi technology. This study demonstrated for the first time that CSBV infection can cause m6A modification changes in larvae, and comprehensively analyzed the m6A modification pattern of its mRNA, and CSBV infection significantly promoted the expression of (Ac represents , = 0.007), but had no effect on the expression of . It was further confirmed that had a significant negative regulatory effect on CSBV replication ( = 0.0432). These results lay a foundation for further exploration of the role of m6A modification in CSBV infection.
中国囊状幼虫病毒（CSBV）对蜜蜂幼虫（尤其是幼虫）具有高度致死性，并给养蜂业造成了相当大的损失。mRNA的N6-甲基腺嘌呤（m6A）修饰是真核生物中主要的转录后修饰，在病毒感染中发挥作用。然而，m6A修饰在CSBV感染中的作用仍不清楚。在此，我们对感染CSBV和未感染的幼虫进行了m6A-seq高通量测序，以研究宿主全转录组范围的m6A修饰并鉴定m6A修饰的基因。共鉴定出671个变异峰。m6A修饰与mRNA表达的联合分析表明，668个基因的mRNA甲基化修饰与表达水平之间存在显著相关性。证明CSBV感染可导致宿主发生重要的m6A修饰变化。我们通过qPCR检测了CSBV感染对两个甲基化调控基因表达的影响。同时，我们使用RNAi技术验证了两个甲基化调控基因对CSBV复制的影响。本研究首次证明CSBV感染可导致幼虫发生m6A修饰变化，并全面分析了其mRNA的m6A修饰模式，且CSBV感染显著促进了（Ac代表，=0.007）的表达，但对的表达无影响。进一步证实对CSBV复制具有显著的负调控作用（=0.0432）。这些结果为进一步探索m6A修饰在CSBV感染中的作用奠定了基础。
