South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, China.
Curr Microbiol. 2010 Nov;61(5):422-8. doi: 10.1007/s00284-010-9633-2. Epub 2010 Apr 9.
Chinese sacbrood virus (CSBV) is the pathogen of Chinese sacbrood disease, which poses a serious threat to honeybee Apis cerana, and tends to cause bee colony and even the whole apiary collapse. Here we report on prevention of CSBV infection by feeding second instar larvae of A. cerana with specific sequences of CSBV double-stranded RNA (dsRNA). Protection of the bee larvae from CSBV by ingestion of CSBV-derived dsRNA was further demonstrated by quantitative real-time PCR (qRT-PCR) and northern blot analysis. The result provides a potential method to protect A. cerana from CSBV infection.
中国囊状幼虫病毒（CSBV）是中国囊状幼虫病的病原体，对中华蜜蜂（Apis cerana）构成严重威胁，容易导致蜂群甚至整个蜂场崩溃。在这里，我们报告了通过喂食中华蜜蜂二龄幼虫 CSBV 双链 RNA（dsRNA）的特定序列来预防 CSBV 感染。通过定量实时 PCR（qRT-PCR）和 northern blot 分析进一步证明了幼虫通过摄入 CSBV 衍生的 dsRNA 来抵抗 CSBV 的感染。该结果为保护中华蜜蜂免受 CSBV 感染提供了一种潜在的方法。
Zotero 插件