Gamer Jackson, Van Booven Derek, Zarnowski Oskar, Perez Melanie, Frank Joshua, Pangeni Rajendra P, Collado Fanny, Klimas Nancy G, Oltra Elisa, Nathanson Lubov
Institute for Neuro Immune Medicine, Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA.
Dr. J.P. Hussman Institute for Human Genomics, Miller School of Medicine, University of Miami, Miami, FL, USA.
Methods Mol Biol. 2025;2920:103-112. doi: 10.1007/978-1-0716-4498-0_7.
Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a chronic, complex multi-organ illness characterized by unexplained debilitating fatigue and post-exertional malaise (PEM). We evaluated transcriptomic changes in peripheral blood mononuclear cells (PBMC) of ME/CFS patients undergoing an exercise challenge and explored the transcriptomic response to exercise and recovery in PBMC of ME/CFS patients, as compared to healthy controls using RNA sequencing technology. As transcriptomic changes in ME/CFS patients are still in the phase of discovery, analysis of data has to be stringent, and the most important results have to be validated by a different technology, such as real-time PCR or NanoString.
肌痛性脑脊髓炎/慢性疲劳综合征（ME/CFS）是一种慢性、复杂的多器官疾病，其特征为不明原因的使人衰弱的疲劳和运动后不适（PEM）。我们使用RNA测序技术评估了接受运动挑战的ME/CFS患者外周血单核细胞（PBMC）的转录组变化，并探讨了与健康对照相比，ME/CFS患者PBMC对运动和恢复的转录组反应。由于ME/CFS患者的转录组变化仍处于发现阶段，数据的分析必须严格，最重要的结果必须通过实时PCR或NanoString等不同技术进行验证。
