Sari Nastiti Intan Permata, Yuliandari Putu, Pramono Ajeng Kusumaningtyas, Tyastuti Dwi, Furqoni Abdul Hadi, Fitriana Fitriana, Sunarno Sunarno, Setyowati Dewi, Dharmayanti Ni Luh Putu Indi
Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency (BRIN), Cibinong Science Centre, Bogor, West Java, Indonesia.
Department of Community and Family Medicine, Faculty of Medicine, Universitas Islam Negeri Syarif Hidayatullah, Jakarta, Indonesia.
BMC Res Notes. 2025 May 15;18(1):218. doi: 10.1186/s13104-025-07269-1.
Klebsiella pneumoniae is a free-living bacterium found anywhere, including soil, water, and various types of plants, animals, and humans. Due to identical biochemical test results, Klebsiella quasipneumoniae, a member of the Klebsiella pneumoniae complex, is often misidentified as Klebsiella pneumoniae. This distinct species can be accurately identified solely through whole-genome sequencing. This bacterium poses a significant public health threat due to its increasing antibiotic resistance, ability to cause severe disease, and potential for community-acquired and hospital-acquired infections. However, there was no previous report of K. quasipneumoniae from Indonesia. Subsequent research focusing on antimicrobial-resistant gene analysis, virulence determinants, evolutionary relationship, and transmission pathways based on this dataset will enhance understanding of this species and their differences with other Klebsiella pneumoniae complex organisms.
We present a whole genome sequencing of four Klebsiella quasipneumoniae isolated from hospital wastewater in Jakarta, Indonesia. Initial bacterial identification was conducted which showed Klebsiella pneumoniae. However, the whole genome Average Nucleotide Identity (wgANI) was found to be Klebsiella quasipneumoniae. The genome size of Klebsiella quasipneumoniae I1-1, I1-3, I5-2, and I5-3 isolates were 5.4 Mb (GC = 57.72%), 5.5 Mb (GC = 57.72%), 5.4 Mb (GC = 57.73%), and 5.5 Mb (GC = 57.72%), respectively. Sequence data has been deposited in the GenBank database.
肺炎克雷伯菌是一种自由生活的细菌，可在任何地方发现，包括土壤、水以及各种类型的植物、动物和人类。由于生化测试结果相同，肺炎克雷伯菌复合体的成员准肺炎克雷伯菌常被误鉴定为肺炎克雷伯菌。仅通过全基因组测序才能准确鉴定这种独特的物种。这种细菌因其不断增加的抗生素耐药性、导致严重疾病的能力以及社区获得性和医院获得性感染的可能性，对公共卫生构成了重大威胁。然而，此前尚无来自印度尼西亚的准肺炎克雷伯菌的报告。基于该数据集，后续聚焦于抗菌耐药基因分析、毒力决定因素、进化关系和传播途径的研究，将增进对该物种及其与其他肺炎克雷伯菌复合体生物差异的理解。
我们展示了从印度尼西亚雅加达医院废水中分离出的4株准肺炎克雷伯菌的全基因组测序结果。最初进行的细菌鉴定显示为肺炎克雷伯菌。然而，全基因组平均核苷酸同一性（wgANI）结果表明是准肺炎克雷伯菌。准肺炎克雷伯菌I1 - 1、I1 - 3、I5 - 2和I5 - 3分离株的基因组大小分别为5.4 Mb（GC = 57.72%）、5.5 Mb（GC = 57.72%）、5.4 Mb（GC = 57.73%）和5.5 Mb（GC = 57.72%）。序列数据已存入GenBank数据库。
